摘要
目的探究安胰颗粒对左旋精氨酸诱导的SAP大鼠胰腺组织NF-κB、iNOS、COX-2表达的影响。方法120只SD大鼠随机分为正常组、模型组、IL-10干预组、安胰颗粒组(8 g/kg)。给药组预给药3 d,左旋精氨酸诱导SAP模型。HE染色观察胰腺组织病理变化,RT-PCR测定胰腺NF-κB mRNA表达,免疫组化检测iNOS、COX-2表达。结果左旋精氨酸诱导SAP模型3、6、12 h后,模型组胰腺组织NF-κB mRNA、iNOS及COX-2表达均升高(P<0.01);经IL-10及安胰颗粒干预后,NF-κB mRNA、iNOS及COX-2表达降低(P<0.05);安胰颗粒组与IL-10干预组比较,差异无统计学意义。结论安胰颗粒能有效抑制NF-κB mRNA、iNOS及COX-2的表达,是其治疗重症急性胰腺炎的机制之一。
AIM To investigate the effects of Anyi Granules on the expression of NF-κB,iNOS and COX-2 in the pancreas tissue of L-arginine-induced severe acute pancreatitis(SAP)rats.METHODS One hundred and twenty SD rats were randomly divided into normal group,model group,IL-10 intervention group,and Anyi Granules group(8 g/kg).L-arginine induced SAP models were established,and 3-day corresponding dosing upon groups later demanding futher interventions was practiced.The rats were subjected to observation of the pathological pancreas changes by HE staining,the determination of pancreatic expression of NF-κB mRNA by RT-PCR,and the detection of iNOS and COX-2 expression by immunohistochemistry.RESULTS The overall increased pancreatic expression of NF-κB mRNA,iNOS and COX-2 in the model group was observed at 3,6,and 12 h after L-arginine induction(P<0.01).The two groups intervened with either IL-10 or Anyi Granules shared decreased expression of NF-κB mRNA,iNOS and COX-2(P<0.05);and were observed with no statistically significant difference.CONCLUSION Anyi Granules highlight the SAP treatment due to their effective inhibition on the expression of NF-κB mRNA,iNOS and COX-2.
作者
文玲
边志远
廖健思
尹胡海
田玉玲
雷力民
WEN Ling;BIAN Zhi-yuan;LIAO Jian-si;YIN Hu-hai;TIAN Yu-ling;LEI Li-min(Guangxi University of Chinese Medicine,Nanning 530001,China;Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,China)
出处
《中成药》
CAS
CSCD
北大核心
2020年第8期2043-2048,共6页
Chinese Traditional Patent Medicine
基金
广西自然科学基金项目(2015jjAA40320)
全国名老中医传承工作室(雷力民名老中医传承工作室)建设项目(2018)
广西中医药大学研究生创新课题(YCSY2018043)。
