摘要
目的:探讨CircAKT3对视网膜母细胞瘤细胞SO-Rb50增殖和凋亡的作用及生物学行为的影响。方法:采用qRT-PCR检测视网膜母细胞瘤细胞系SO-Rb50和视网膜上皮细胞细胞系ARPE-19中CircAKT3的表达水平。后续将SO-Rb50细胞分为两组,实验组细胞转染CircAKT3 SiRNA,而对照组转染SiRNA对照序列。采用qRT-PCR检测细胞的转染效率,采用CCK-8实验检测两组细胞的增殖能力,采用流式细胞仪检测两组细胞的凋亡率。结果:相较于ARPE-19细胞,SO-Rb50细胞内CircAKT3的表达水平明显增高(P<0.01)。实验组在转染CircAKT3 SiRNA 24 h后,细胞内CircAKT3的表达水平较对照组明显降低(P<0.05)。CCK-8实验结果显示,实验组在24、48 h的OD值和对照组无统计学差异,而在72、96 h的OD值明显低于对照组(P<0.05)。流式细胞仪结果显示,实验组和对照组的凋亡率分别为(7.71±0.396)%和(3.51±0.478)%,实验组凋亡率明显高于对照组(P<0.001)。结论:CircAKT3可促进SO-Rb50细胞的增殖和抑制细胞凋亡。
Objective:To explore the effect of CircAKT3 on the proliferation and apoptosis of retinoblastoma SO-Rb50 cells,so as to study the effect of CircAKT3 on biological behaviors of retinoblastoma cells.Methods:The expression of CircAKT3 in retinoblastoma SO-Rb50 cells and retinal epithelial ARPE-19 cells was deteced by qRT-PCR.Subsequently,SO-Rb50 cells were divided into two groups.Cells of the experimental group were transfected with CircAKT3 siRNA,while those of the control group transfected with siRNA control sequence.The transfection efficiency was detected by qRT-PCR.CCK-8 assay was used to detect the ability of cell proliferation of the two groups.flow cytometry was used to detect the apoptosis rate.Results:Compared with ARPE-19 cells,the expression of CircAKT3 in SO-Rb50 cells was significantly increased(P<0.01).24 hours after transfection with CircAKT3 siRNA in the experimental group,the expression of CircAKT3 in the cells was significantly lower than that in the control group(P<0.05).The results of CCK-8 assay showed that OD values of the experimental group at 24 and 48 hours were not different from those of the control group,while OD values at 72 and 96 hours were significantly lower than those of the control group(P<0.05).The results of flow cytometry showed that the apoptosis rate of the experimental group and the control group were(7.71±0.396)%and(3.51±0.478)%,respectively,and the apoptosis rate of the experimental group was significantly lower than that of the control group(P<0.001).Conclusion:CircAKT3 can promote the proliferation of SO-Rb50 cells and inhibit cell apoptosis.
作者
周鹏翔
信波
宋晓鹏
孙启会
ZHOU Pengxiang;XIN Bo;SONG Xiaopeng(Tai’an Medical District,the 960th Hospital of PLA,Tai’an 271100)
出处
《陕西医学杂志》
CAS
2020年第8期932-935,共4页
Shaanxi Medical Journal
