摘要
目的[HT5"SS]探讨骨膜蛋白(Periostin)在急性创伤性脑损伤(aTBI)大鼠中的水平,并分析其作用机制,为进一步研究急性创伤性脑损伤发病机制及治疗提供具有临床价值的信息。方法取清洁级大鼠36只,构建急性脑损伤手术模型,成功后将其随机分成A、B、C组,每组各12只大鼠。A组为空白对照健康大鼠,B组为aTBI大鼠,C组为接受Periostin干预的aTBI大鼠。每组6只于建模后第24h用于Periostin浓度、认知、行为能力及炎性因子检测;另外6只于颅脑损伤后24h断头处死,取出脑挫伤灶制作病理标本,染色后采用免疫组化法检测神经细胞形态。结果C组外周血、脑脊液Periostin浓度高于B组和A组(P<0.05);B组脑脊液Periostin浓度高于外周血浓度(P<0.05);3组IL-6、TNF-α、CRP水平无统计学意义(P>0.05);3组神经功能缺损程度评分(NFDS)水平比较差异有统计学意义(P<0.05);C组神经细胞凋亡率高于B组和A组(P<0.05);C组对特异性蛋白微管结合蛋白2(MAP2)、基质金属蛋白酶-2(MMP-2)表达高于B组和A组。结论Periostin来自中枢神经系统,可通过加快神经细胞凋亡、抑制特异性蛋白表达进一步导致脑损伤恶化,应在TBI治疗中多关注Periostin浓度变化。
Objective To investigate the expression and mechanism of Periostin in rats with acute traumatic brain injury(aTBI),and to provide a valuable information for the further study on pathogenesis and treatment of aTBI.Methods 36 clean-grade rats were randomly divided into groups A,B and C and 12 rats in each group.Group A received the sham-operation,group B was made into aTBI model without intervention,while group C was made into aTBI model with Periostin intervention.The Periostin concentration,cognitive,behavioral abilities and inflammatory factors after modeling 24 hours were detected in each group with six rats.The other 6 were sacrificed with severed heads 24 hours after craniocerebral injury.The lesions of brain contusion were removed to make pathological specimens.After staining,the morphology of nerve cells was detected by immunohistochemical method.Results The Periostin concentration in peripheral blood and cerebrospinal fluid were higher than that in group A and group B(P<0.05).In group B,Periostin concentration in cerebrospinal fluid was higher than that in peripheral blood(P<0.05).There was no significance difference in IL-6,TNF-αand CRP among the three groups(P>0.05).There were significant differences in NFDs among the three groups(P<0.05).The apoptotic rate of neurons,the expression levels of specific protein microtubule-associated protein 2 and matrix metalloproteinase-2 among three groups in a descending order were group C,group B,and group A(P<0.05).Conclusion Periostin is derived from the central nervous system and it can further aggravate brain damage by accelerating neuronal apoptosis and inhibiting specific protein expression.So the monitor of the Periostin concentration could be strengthened in the treatment of TBI.
作者
王华
凌云
杨欣
WANG Hua;LING Yun;YANG Xin(Emergency Department,Shanghai Pudong Public Hospital,Shanghai 200135,China)
出处
《宁夏医学杂志》
CAS
2020年第7期584-587,I0001,共5页
Ningxia Medical Journal
关键词
骨膜蛋白
急性创伤性脑损伤
表达
临床意义
Periostin
Acute traumatic brain injury
Expression
Clinical significance
