摘要
目的:通过建立孕期大鼠尼古丁暴露模型,并在孕期进行电针足三里治疗,观察围产期子代宫内生长迟缓(IUGR)、新生大鼠肺脏发育的变化以及血浆促肾上腺皮质激素(ACTH)和皮质酮(CORT)的改变,探讨尼古丁暴露所致发育不良的可能机制。方法:将120只雌性孕期SD大鼠随机分为空白对照组、模型组和电针组,每组各40只。模型组和电针组从孕期第6天至分娩前给予呼吸道静式染毒,对照组大鼠同时放置于染毒箱内,但不进行染毒处理。造模当日电针组大鼠经束缚后进行电针足三里治疗,模型组与对照组大鼠仅进行束缚,不做任何治疗。所有大鼠均自然分娩,收集各组大鼠分娩新生鼠数量和体重,计算IUGR的发生率。经21 d哺乳后,采用动物肺功能分析系统对三组新生鼠肺功能进行测定和比较,并对孕期尼古丁摄入量与新生鼠体重、肺指数的相关性进行分析。结果:分娩后,空白对照组雌鼠分娩新生鼠7~10只,平均(8.78±1.25)只;模型组雌鼠分娩新生鼠4~8只,平均(6.71±0.95)只;电针组雌鼠分娩新生鼠6~9只,平均(7.96±1.25)只,组间比较雌鼠分娩新生鼠数量差异有统计学意义(P<0.05)。其中,模型组雌鼠分娩新生鼠数量相比电针组、空白对照组均降低(P<0.05),电针组相比对照组降低(P<0.05),但各组新生鼠雌雄分布差异无统计学意义(P>0.05)。体重方面,空白对照组新生小鼠体重5.8~9.0 g,平均体重(7.33±1.14)g,模型组新生小鼠体重3.4~8.3 g,平均体重(6.34±1.58)g,IUGR 25例,发生率为26.60%,电针组新生小鼠体重4.5~8.6 g,平均体重(7.09±1.25)g,IUGR 20例,发生率为8.97%。组间比较,三组新生鼠体重差异有统计学意义(P<0.05),其中模型组新生鼠体重与电针组、空白对照组相比均降低(P<0.05),IUGR发生率相比电针组提高(P<0.05)。而电针组与空白对照组在新生鼠体重方面比较,差异无统计学意义(P>0.05)。三组新生鼠分娩21 d后,PIF、PEF、MV以及肺指数比较,差异均有统计学意义(P<0.05);模型组、电针组PIF、PEF、MV以及肺指数相比空白对照组均降低,电针组PIF、PEF、MV以及肺指数相比模型组增高,差异有统计学意义(P<0.05)。经Pearson相关性分析显示,模型组、电针组新生鼠出生体重与肺指数均呈正相关(P<0.05)。治疗后,三组大鼠间的血浆ACTH、CORT水平差异有统计学意义(P<0.05);模型组、电针组大鼠血浆ACTH、CORT水平明显高于空白对照组(P<0.05);电针组大鼠血浆ACTH、CORT水平明显低于模型组(P<0.05)。结论:对尼古丁暴露孕期大鼠给予电针足三里治疗可有效降低新生鼠IUGR的发生率,且有利于改善新生鼠的肺功能。
Objective:Though establishing the rat model of nicotine exposure during pregnancy,and cupping ST36 therapy during pregnancy,observe the perinatal children Intrauterine Growth Retardation(IUGR)and the change of lung development and plasma Adrenocorticotrophic hormone(ACTH)and corticocorticosterone(CORT)in the newborn rat,explore the improvement of nicotine exposure caused by the method of prevention and cure of neonatal dysplasia.Methods:120 pregnant female DS rats were randomly divided into blank control group,model group and electroacupuncture group,40 cases in each group.The model group and the electroacupuncture group were given respiratory static infection,while the rats in the control group were placed in the contaminated box at the same time,but not treated.On the day of moulding,the rats in the electroacupuncture group were treated with ST36 electroacupuncture after being bound,while the rats in the model group and the control group were only bound without any treatment.All rats gave birth naturally,and the number and weight of newborn rats in each group were collected to calculate the incidence of IUGR.After 21 days of lactation,the lung function of the three groups was measured and compared by the animal lung function analysis system,and the correlation between nicotine intake during pregnancy and neonatal rats'body weight and lung index was analyzed.Results:After childbirth,females in blank control group gave birth to new mice 7~10,the average was(8.78±1.25),femal in model group gave birth to new mice 4~8,the average was(6.71±0.95),the curative group of female gave birth to new mice 6~9,the average was(7.96±1.25),compared the difference of female labor number of newborn mice between groups had statistical significance(P<0.05).The number of newborn rats in the model group was lower than that in the electroacupuncture group and the blank control group(P<0.05),and that in the electroacupuncture group was lower than that in the control group(P<0.05),but there was no statistically significant difference in the distribution of male and female newborn mice in each group(P>0.05).In terms of body weight,newborn mice in the blank control group weighed was 5.8~9.0 g,with an average body weight of 7.33±1.14 g,newborn mice in the model group weighed 3.4~8.3 g,with an average body weight of 6.34±1.58 g,and with 25 cases of IUGR,the incidence of 26.60%,newborn mice in the electroacupuncture group weighed 4.5~8.6 g,the average body weight was 7.09±1.25 g,and with 20 cases of IUGR,the incidence of 8.97%,there was significant difference in body weight among the three groups(P<0.05).The weight of neonatal mice in the model group was significantly lower than that in the electroacupuncture group and the blank control group(P<0.05),and the incidence of IUGR was significantly higher than that in the electroacupuncture group(P<0.05).However,there was no significant difference between the electroacupuncture group and the blank control group in the weight of newborn mice(P>0.05).21 days after delivery,the differences in PIF,PEF,MV and lung index in neonatal rats between the groups were statistically significant(P<0.05).PIF,PEF,MV and lung index of the model group and the electroacupuncture group were all decreased than those in the blank control group,while PIF,PEF,MV and lung index of the electroacupuncture group were all increased than those in the model group,with statistically significant differences(P<0.05).Pearson correlation analysis showed that there was a positive correlation between birth weight and lung index in the model group and the electroacupuncture group(P<0.05).After treatment,the differences in plasma ACTH and CORT levels between the three groups were statistically significant(P<0.05).The plasma ACTH and CORT levels of rats in the model group and electroacupuncture group were significantly higher than those in the blank control group(P<0.05).Plasma levels of ACTH and CORT in the electroacupuncture group were significantly lower than those in the model group(P<0.05).Conclusion:The incidence of IUGR in newborn mice can be effectively reduced by zusanli electroacupuncture in pregnant rats exposed to nicotine,and the lung function of newborn mice can be improved.
作者
刘兆敏
唐琴
袁林
张生玉
彭芬
LIU Zhao-min;TANG Qin;YUAN Lin;ZHANG Sheng-yu;PENG Fen(Department of Neonatology,En'shi 445000,Hubei,China;Occurrence and Intervention of Rheumatic Diseases in the Key Laboratory of Hubei Province,Hubei Enshi Hualong General Hospital,En'shi 445000,Hubei,China;Department of Rehabilitation Medicine,Affiliated Minda Hospital of Hubei University for Nationalities,Hubei Enshi Hualong General Hospital,En'shi 445000,Hubei,China;Department of Neonatology,Hubei Enshi Hualong General Hospital,En'shi 445000,Hubei,China)
出处
《川北医学院学报》
CAS
2020年第4期588-592,共5页
Journal of North Sichuan Medical College
基金
湖北省教育厅科研课题(D20171901)。
关键词
尼古丁
电针
足三里
子代大鼠
宫内生长受限
肺功能
Nicotine
Electric acupuncture
St 36
Offspring rats
Intrauterine growth restriction
Lung function
