摘要
目的:研究黄芪对肝纤维化大鼠肝损伤的保护作用及相关机制。方法:将SD大鼠随机分为正常对照组、肝纤维化模型组、黄芪后处理组和阳性对照组,检测各组大鼠血清中ALT、AST活性和TBIL水平变化;逆转录-聚合酶链反应检测各组大鼠肝脏组织5种主要致纤维化因子p38MAPK、TGF-β1、α-SMA、CTGF和CollegenⅣmRNA表达水平;免疫印迹法检测p38MAPK信号通路上下游蛋白MKK3和ATF-2的表达变化。结果:与肝纤维化模型组比较,黄芪后处理组大鼠血清中ALT、AST和TBIL水平明显降低(P<0.05),肝纤维病理变化明显减轻,p38MAPK、MKK3和ATF-2蛋白表达量均降低(P<0.05)。结论:黄芪通过p38MAPK信号通路对实验性肝纤维化大鼠肝损伤具有效的保护作用。
Objective:To study the protective effect of astragalus on liver injury in rats with liver fibrosis and its mechanism.Methods:SD rats were randomly divided into normal control group,liver fibrosis model group,astragalus post-treatment group and positive control group.Serum ALT,AST and TBIL levels in each group were detected.Five main fibrogenic factors:p38MAPK,TGF-β1、α-SMA,CTGF and CollegenⅣmRNA expression in rat liver tissue between groups were detected by RT-PCR.Western blotting was used to detect the expression changes of MKK3 and ATF-2 proteins in the upstream and downstream p38MAPK signaling pathway.Results:Compared with the liver fibrosis model group,the serum levels of ALT,AST and TBIL in the astragalus post-treatment group were significantly reduced(P<0.05).The pathological changes of liver fibers were significantly reduced,and the protein expressions of p38MAPK,MKK3 and ATF-2 were significantly reduced(P<0.05).Conclusion:Astragalus has an effective protective effect on liver injury in rats with experimental liver fibrosis through the p38MAPK signaling pathway.
作者
雷玲
闵珺
刘锋
肖秀清
杜凡
LEI Ling;MIN Jun;LIU Feng(The Third Affiliated Hospital of Nanchang University,Nanchang 330008)
出处
《陕西中医》
2020年第9期1192-1196,共5页
Shaanxi Journal of Traditional Chinese Medicine
基金
江西省卫生健康委员会科技计划项目(20184004)。
