摘要
目的:建立同时测定茯苓药材中7种三萜类成分含量的方法,并比较不同产地茯苓药材中上述成分的差异性,为茯苓药材的质量控制提供参考。方法:以不同产地的36批茯苓药材为样品,采用高效液相色谱法测定去氢土莫酸、猪苓酸C、3-表去氢土莫酸、3-O-乙酰基-16α-羟基-氢化松苓酸、去氢茯苓酸、茯苓酸、松苓新酸的含量。色谱柱为Thermo Acclaim 120 C18,流动相为乙腈-磷酸水(梯度洗脱),流速为1 mL/min,检测波长为210 nm,柱温为30℃,进样量为20μL。采用SPSS 21.0统计学软件对36批不同产地茯苓药材进行聚类分析。结果:7种三萜类成分在各自质量浓度范围内线性关系均良好(r均不低于0.9990),平均加样回收率为96.74%~104.04%(RSD为0.54%~1.55%,n=6);精密度、重复性、稳定性(24 h)试验的RSD均小于3.0%(n=6);耐用性试验的RSD均小于5.0%(n=2)。不同产地样品之间7个指标成分的单一含量均存在一定差异,但总体差异不明显(多数样品总含量分布在1.3~1.9 mg/g之间)。经聚类分析,36批样品共聚为5类,即S27单独聚为第Ⅰ类,S30、S34聚为第Ⅱ类,S2、S8、S9聚为第Ⅲ类,S10、S11、S12、S14聚为第Ⅳ类,其余26批样品聚为第Ⅴ类。结论:该方法操作简单,精密度、重复性、耐用性均良好,可用于同时测定茯苓药材中上述7种三萜类成分的含量。各产地茯苓药材的质量总体差异不显著,大部分产区的茯苓含量均一、质量稳定,仅少部分存在差异。
OBJECTIVE:To establish a method for the content determination of 7 kinds of triterpenoids in Poria cocos,and to compare the differences of the above components in P.cocos from different habitats,so as to provide reference for the quality control of P.cocos.METHODS:Using 36 batches of P.cocos from different habitats as samples,HPLC method was used for content determination of dehydrotomorphic acid,polyporhinic acid C,3-epidehydrotomorphic acid,3-O-acetyl-16α-hydroxyhydrogenolysaccharic acid,dehydrotomorphic acid,pachymic acid and dehydrotrametenolic acid.The column was performed on Thermo Acclaim 120 C18 with mobile phase consisted of acetonitrile-phosphoric acid water(gradient elution)at the flow rate of 1 mL/min.The detection wavelength was set at 210 nm.The column temperature was 30℃,and the injection volume was 20μL.SPSS 21.0 statistical software was used for cluster analysis of 36 batches of P.cocos from different habitats.RESULTS:The linearity of 7 triterpenoids was good in the range of their mass concentration(all r≥0.9990);average recoveries were 96.74%-104.04%(RSD=0.54%-1.55%,n=6).RSDs of precision,and reproducibility stability(24 h)tests were all lower than 3.0%(n=6).RSD of durability test was lower than 5.0%(n=2).There were some differences in the single content of 7 indicator components among samples from different habitats,but the total content difference was not obvious(the total content of most samples was in the range of 1.3-1.9 mg/g).After cluster analysis,36 batches of sample were clustered into 5 categories,i.e.S27 was clustered into classⅠ;S30 and S34 were clustered into classⅡ;S2,S8 and S9 were clustered into classⅢ;S10,S11,S12 and S14 were clustered into classⅣ;and the remaining 26 batches of samples were clustered into classⅤ.CONCLUSIONS:The method is simple,and has good precision,repeatability and durability.It can be used for the simultaneous determination of above 7 components in P.cocos.There has no significant difference in the quality of P.cocos from different habitats.The content of P.cocos in most producing areas is uniform in content and stable in quality,only a few of them are different.
作者
万鸣
黄超
杨玉莹
罗心遥
叶晓川
丁影
董琼
陈树和
WAN Ming;HUANG Chao;YANG Yuying;LUO Xinyao;YE Xiaochuan;DING Ying;DONG Qiong;CHEN Shuhe(Dept,of Pharmaceutical Affairs,Hubei Province Hospital of TCM/Hubei Academy of TCM,Wuhan 430061,China;College of Pharmacy,Hubei University of TCM,Wuhan 430065,China)
出处
《中国药房》
CAS
北大核心
2020年第17期2101-2106,共6页
China Pharmacy
基金
国家重点研发计划中医药现代化研究重点专项(No.2017YFC1703000)
湖北省技术创新专项(No.2019ACA121)。
