摘要
目的观察针刺对自身免疫性脑脊髓炎小鼠(EAE)的疗效及其对脑干磷酸化丝裂原活化蛋白激酶(p-p38MAPK)的影响,探究针刺对EAE小鼠的治疗作用和机制,为临床治疗MS提供新的思路方法。方法将40只C57BL/6小鼠随机分成4组。空白组小鼠不作处理,其余组采用髓鞘少突胶质细胞糖蛋白35-55(MOG35-55)制备的完全抗原将小鼠诱导成EAE模型。造模后第14天开始,空白组及模型组每日固定,并用生理盐水灌胃。激素组每日与醋酸泼尼松灌胃。针刺组小鼠针刺"大椎"、双侧"肾俞""足三里"。记录小鼠体重和神经功能评分。免疫第28天后对脑组织进行取材,用LFB染色观察病理变化,检测脑组织中p-p38MAPK免疫组化表达及p-p38MAPK的WB表达。结果与空白组相比,其余各组小鼠体重降低(P<0.05),神经功能评分升高(P<0.05),LFB染色脱髓鞘程度升高,p-p38MAPK免疫组化及WB检测均升高(P<0.05)。与模型组相比,针刺组及激素组体重升高(P<0.05),神经功能评分降低(P<0.05),LFB染色脱髓鞘程度降低,p-p38MAPK免疫组化及WB检测均降低(P<0.05)。与激素组相比,针刺组体重降低(P<0.05),神经功能评分升高(P<0.05),p-p38MAPK免疫组化及WB检测升高,但差异无统计学意义(P>0.05)。结论针刺可能是通过降低EAE小鼠p-p38MAPK表达,降低EAE小鼠炎症反应,减轻神经损伤从而发挥治疗作用。
Objective To observe the effect of acupuncture on experimental autoimmune encephalomyelitis(EAE)mice and the effect on the expressions of brainstem phosphorylated P38 mitogen-activated protease,and to explore the effect and mechanism of acupuncture on EAE mice,and to provide the new idea and method for treatment of MS.Methods 40 mice of C57 BL/6 were randomly divided into 4 groups.EAE model was established in C57 BL/6 mice by complete antigen prepared from MOG35-55 except for the blank group.Since the 14 th day after modeling,the blank group and the model group were fixed and given by gavage with physiological saline every day.The hormone group were given by gavage with prednisone.Acupuncture group received acupuncture at"Dazhui"point,bilateral"Shenshu"point,bilateral"Zusanli"point.Recording the weight and neurological function score of each group.On the 28 th day,the pathological changes in brain tissue were observed by Luxol Fast Blue(LFB)myelin staining method,the expressions of p-p38 MAPK in brain tissue were measured by immunohistochemistry method and western blot.Results Compared to the blank group,the weights of the other groups were lower(P<0.05);the neurological function scores were higher(P<0.05);the degrees of demyelination were higher;the expressions of p-p38 MAPK by immunohistochemical analysis and western blot test were higher(P<0.05).Compared to the model group,the weights of the acupuncture group and hormone group were higher(P<0.05);the neurological function scores were lower(P<0.05);the degrees of demyelination were lower;the expressions of p-p38 MAPK by immunohistochemical analysis and western blot test were lower(P<0.05).Compared to the hormone group,the weight of the acupuncture group was lower(P<0.05);the neurological function score was higher(P<0.05);the expressions of p-p38 MAPK by immunohistochemical analysis and western blot test were higher,but with no statistical difference(P>0.05).Conclusion Acupuncture can reduce the inflammation of EAE mice and alleviate the extent of nerve damage.It may be related to lowering the expression of pp38 MAPK.
作者
宋方亚
辛祺亮
赵东杰
李琪
王玉华
董莎
辛随成
Song Fangya;Xin Qiliang;Zhao Dongjie;Li Qi;Wang Yuhua;Dong Sha;Xin Suicheng(Beijing University of Chinese Medicine,Beijing 100029,China;Beijing University of Chinese Medicine Dongfang College,Langfang 065001,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2020年第6期2080-2086,共7页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
