摘要
目的:通过实验研究,探讨慢咽膏对慢性咽炎的疗效机制。方法:采用小鼠毛细血管通透性实验、大鼠棉球肉芽肿实验,评价慢咽膏的抗炎作用;采用氨水诱导小鼠咳喘,记录小鼠咳喘潜伏期及咳喘次数,评价慢咽膏的止咳作用;用氨水制备慢性咽炎大鼠模型,取咽喉部组织行苏木精-伊红(Hematoxylin-eosin Staining,HE)染色法染色,镜下观察慢咽膏对慢性咽炎大鼠咽喉部病变组织的治疗保护作用。结果:毛细血管通透性实验结果显示,慢咽膏高剂量组腹腔灌洗液OD值相对于低、中剂量组显著性降低(P<0.05);咳嗽抑制实验结果显示,慢咽膏中剂量组的咳喘潜伏期及抑制程度相对于低、高剂量组明显较长(P<0.05),咳喘次数显著性减少(P<0.05);棉球肉芽肿实验结果显示,与对照组相比,阿司匹林组、慢咽膏高、中、低剂量组的肉芽肿干重及肉芽肿重与100 g体质量的比值均显著性下降(P<0.01);慢性咽炎模型大鼠咽部组织病理观察结果显示,慢咽膏高、中、低剂量组均能显著改善慢性咽炎模型大鼠咽喉壁炎症病变。结论:慢咽膏具有一定的消炎镇咳作用,对慢性咽炎有较好的治疗作用。
Objective:To explore efficacy of the Manyan cream(慢咽膏)on chronic pharyngitis.Methods:The anti-inflammatory effect of Manyan cream was evaluated by capillary permeability test in mice and cotton ball granuloma test in rats.Cough and asthma in mice were induced by ammonia,the latent period and times of cough were recorded,and the antitussive effect of the Manyan cream was evaluated.The chronic pharyngitis rat models were established,then effects of the Manyan cream on the pathology of pharyngeal tissue was observed.Results:The capillary permeability test showed that the OD value in the high dose group was significantly lowest(P<0.05).The latent period of cough and asthma was significantly longest(P<0.05),and the times of cough and asthma were most decreased in the middle dose group(P<0.05).The cotton ball granuloma test showed that the dry weight of granuloma and the ratio of granuloma weight to 100 g body weight in the aspirin group,high dose group,middle dose group and low dose group were significantly lower than those in control group(P<0.01).The pathological observation of the pharyngeal tissues of the chronic pharyngitis model rats showed that the inflammation of the throat wall in the pharyngitis model rats was improved in the high,middle and low dose groups.Conclusion:The Manyan cream has certain anti-inflammatory and antitussive effects,and was effective on chronic pharyngitis.
出处
《中医临床研究》
2020年第23期1-4,共4页
Clinical Journal Of Chinese Medicine
基金
广东省中医药局-中医药强省立项(项目编号:20152031)。
关键词
慢咽膏
抗炎
止咳
机制研究
The Manyan cream
Anti-inflammation
Relieving a cough
A mechanism study
