摘要
目的:观察紫草素对人食管癌TE-1细胞增殖、凋亡和细胞周期的影响,并探究其作用机制。方法:采用不同浓度的紫草素(0、1、5、10µmol/L)处理TE-1细胞,MTT法检测24、48、72 h后各组细胞增殖水平;紫草素处理各组TE-1细胞48 h后,采用Hoechst 33258荧光染色观察细胞凋亡状况,流式细胞术检测细胞的凋亡水平及周期变化,Western blotting检测TRAP1/Akt/mTOR信号通路相关蛋白表达变化。结果:紫草素呈时间和浓度依赖性抑制TE-1细胞增殖(P<0.05或P<0.01);与对照组相比,紫草素能显著促进TE-1细胞凋亡(P<0.01),使TE-1细胞周期发生G0/G1期阻滞(P<0.05或P<0.01),同时降低TRAP1、p-Akt以及p-mTOR表达水平(P<0.05或P<0.01),以上作用具有剂量依赖性。结论:紫草素能显著抑制TE-1细胞的增殖,诱导细胞发生G0/G1期阻滞并促进其凋亡,这可能与其抑制TRAP1/Akt/mTOR信号通路有关。
Objective:To observe the effects of shikonin on the proliferation,apoptosis and cell cycle of human esophageal carci‐noma TE-1 cells,and to explore its mechanism.Methods:TE-1 cells were treated with different concentrations of shikonin(0,1,5,10µmol/L).MTT assay was used to detect cell proliferation at different time points(24,48 and 72 h).After treatment with shi‐konin for 48 h,cell apoptosis in TE-1 cells of each group was observed with Hoechst 33258 fluorescence staining.Flow cytometry was used to detect apoptosis and cell cycle.The changes in expression of TRAP1/Akt/mTOR signaling pathway related proteins were detected by Western blotting.Results:Shikonin inhibited the proliferation of TE-1 cells in a time-dose-dependent manner(P<0.05 or P<0.01).Compared with the control group,shikonin significantly promoted the apoptosis of TE-1 cells(P<0.01),induced the G0/G1 phase block of TE-1 cells(P<0.05 or P<0.01),and reduced the expression levels of TRAP1,p-Akt and p-MTOR(P<0.05 or P<0.01).The above effects were all dose-dependent.Conclusion:Shikonin can significantly inhibit the proliferation of TE-1 cells in vitro,induce G0/G1 phase arrest and promote apoptosis,which may be closely related to the inhibition of TRAP1/Akt/mTOR signaling pathway.
作者
赵莉
黄景荣
龚承先
汪毅
屈银宗
计春燕
杨建美
ZHAO Li;HUANG Jingrong;GONG Chengxian;WANG Yi;QU Yinzong;JI Chunyan;YANG Jianmei(Department of Gastroenterology,Hubei Provincial Hospital of Integrated Chinese&Western Medicine,Wuhan 430015,Hubei,China)
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2020年第8期889-894,共6页
Chinese Journal of Cancer Biotherapy
