摘要
在水温(26.0±1.0)℃下,将体质量(0.386±0.080)g的方斑东风螺幼螺置于盐度18、23、28、33(对照组)、38的海水中,6、12、24、36、48、72、96 h后观察幼螺行为,测定其体内6种免疫酶的活性。试验结果显示,方斑东风螺保持良好状态的盐度为20~35,盐度和处理时间显著影响方斑东风螺免疫酶活力(P<0.05)。与对照组相比,盐度18和23处理组幼螺过氧化氢酶活性呈“抑制—诱导”的趋势,而盐度28和38处理组过氧化氢酶活性呈“诱导—抑制—诱导”的变化;各盐度处理组碱性磷酸酶和酸性磷酸酶活性具有相一致的变化规律,均为“诱导—抑制”;盐度38处理组总超氧化物歧化酶活性与同盐度组的过氧化氢酶活性变化一致,而盐度18、23、28处理组的总超氧化物歧化酶活性均表现出“诱导—抑制”的趋势;各盐度处理组谷胱甘肽过氧化物酶活性和盐度18、38处理组的过氧化物酶活性呈现“抑制—诱导—抑制”的趋势,盐度23和28处理组过氧化物酶活性与同盐度总超氧化物歧化酶活性变化一致。
Behavior and activities of six enzymes related to immune were determined in juvenile snail Babylonia areolata with average body weight of(0.386±0.080)g exposed to a salinity of 18,23,28,33(control group)and 38 at water temperature of(26.0±1.0)℃for 6,12,24,36,48,72 and 96 hours to evaluate the changes in activities of the enzymes.The results showed that the suitable salinity for growth of the snail was ranged from 20 to 35,and high mortality was observed at the salinity of below 15 or above 45.Both the increase in salt concentration and in exposure period led to have significant effects on the activities of the immune enzymes(P<0.05).Compared with the control group,the catalase activity showed a“suppression-induction”trend in the snail in salinity 18 and 23 groups,and an“induction-suppression-induction”in the salinity 28 and 38 groups.The consistent“induction-suppression”in acid phosphatase and alkaline phosphatase activities were found in the snail in each salinity group,and the same case was observed in total superoxide dismutase activity of the snail in salinity 38 group,in catalase activity in the same salinity group.The total superoxide dismutase activity showed the trend of“induction-suppression”in salinity 18,23 and 28 groups.The trend of“suppression-induction-suppression”in glutathione peroxidase activity in each salinity group and the peroxidase activity in the salinity 18 and 38 groups were found.The peroxidase activity in salinity 23 and 28 groups was consistent with the total superoxide dismutase activity change in the same salinity.So,the salinity in the aquaculture water will have a greater impact on the growth and immune enzyme activity of the snail.The catalase,alkaline phosphatase,total superoxide dismutase,acid phosphatase and glutathione peroxidase activities were induced when B.areolata were subjected to mild stress,while their activities were inhibited by severe stress.
作者
谭春明
赵旺
吴开畅
温为庚
杨蕊
陈旭
于刚
TAN Chunming;ZHAO Wang;WU Kaichang;WEN Weigeng;YANG Rui;CHEN Xu;YU Gang(Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China;Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China)
出处
《水产科学》
CAS
CSCD
北大核心
2020年第5期677-684,共8页
Fisheries Science
基金
现代农业产业技术体系建设专项(CARS-49)
广东省渔港建设和渔业产业发展专项(A201601B11)
海南省自然科学基金青年基金资助项目(319QN341)
三亚市院地科技合作项目(2018YD19)。
关键词
方斑东风螺
盐度胁迫
免疫酶
Babylonia areolata
salinity stress
enzyme related to immune
