摘要
为了解斑石鲷循环水养殖系统生物滤池内部细菌群落组成及其净水机制,通过高通量测序方法,研究了不同时期各级生物滤池的细菌群落结构,分析了各级生物滤池的水质参数及水处理效果。结果表明:实验筛选出37个门和513个细菌属,第3级生物滤池整体微生物群落丰富度和多样性均高于第1级和第2级生物滤池,第2级和第3级生物滤池微生物群落相似性最高。在门水平上,优势菌为变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、拟杆菌门(Bacteroidetes);在属水平上,发现了起硝化作用的亚硝化单胞菌属Nitrosomonas和硝化螺菌属Nitrospira;实验还发现该系统生物滤池可能存在功能上的浪费现象。该系统的细菌群落结构有稳定的演替模式,生物膜微生物群落变化对水质有一定程度的动态响应,Muricauda、Maribacter等反硝化细菌对硝态氮浓度的变化作用不明显。
In order to learn the bacterial community composition and water purification mechanism in biofilter for recirculating aquaculture system of Oplegnathus punctatus,the high-throughput sequencing method was used to study the evolution of bacterial community structure in the biofilters at different stages,and the water quality parameters and water treatment effects of these biofilters were also analyzed.The results showed that bacterial 37 phyla and 513 genera were screened out by the experiment.The overall microbial community richness and diversity of the third-stage biofilter were higher than those of the first-stage and second-stage biofilters.The microbial communities in the second-stage and third-stage biofilters had the highest similarity.At the phylum level,the dominant bacteria were Proteobacteria,Chloroflexi,Bacteroidetes.At the genus level,Nitrosomonas and Nitrospira were found to perform nitrifying.Morover,some functional waste phenonmena occurred in the biofilter of the system.The analysis indicated that a stable succession pattern existed in the bacterial community structure of this system,and a certain degree of dynamic response of the change of biofilm microbial community to water quality also happened.Denitrifying bacteria such as Muricauda and Maribacter had no obvious effect on the change of nitrate nitrogen concentration.
作者
张皓天
宋协法
刘兴震
董登攀
黄志涛
翟介明
ZHANG Haotian;SONG Xiefa;LIU Xingzhen;DONG Dengpan;HUANG Zhitao;ZHAI Jieming(Fisheries College,Ocean University of China,Qingdao 266003,China;Shandong Laizhou Mingbo Aquatic Products Co.Ltd.,Yantai 264000,China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2020年第8期2176-2185,共10页
Chinese Journal of Environmental Engineering
基金
青岛市高效工厂化循环水养殖关键技术与成套设备研制及产业化应用项目。
关键词
高通量测序
生物滤池
微生物群落
动态响应
循环水养殖(RAS)
high-throughput sequencing
biofilters
bacterial community
dynamic response
recirculating aquaculture system(RAS)
