摘要
为了简化重组大肠杆菌生产番茄红素的工艺,该研究通过选择大肠杆菌中17个转录因子相关基因的启动子序列,以编码番茄红素合成基因的CrtEBI为目的基因,通过构建表达番茄红素合成基因的质粒,来评估转录因子相关基因启动子序列对番茄红素产量的影响。通过比较不同菌株合成番茄红素的能力,确定最佳启动子。结果表明,含有组成型启动子P lpp或P mglb的菌株合成番茄红素的能力较强,相较于传统诱导型启动子菌株产量分别提高了347.7%和51.3%。由于筛选到的启动子为组成型启动子,省去了添加诱导剂的过程,达到了优化生产工艺、降低生产成本的目的。
Lycopene is a kind of fat-soluble carotenoids with strong antioxidant properties.It is widely used in food,medicine and other industries.Previous studies have constructed engineered Escherichia coli with high-level production of lycopene using inducer.But these methods are labor intensive and time-consuming.In order to simplify the lycopene production,17 promoters that regulated by transcription factor in E.coli were selected and E.coli recombinants were constructed e by cloning promoter sequences and ligating with report gene crtEBI.E.coli recombinant with the promoter P lpp and P mglb were mediated more effective expression.The lycopene yield increased by 51.3%compared with the control strain pTRC99aEBI.
作者
杨帆
苏卜利
王永红
张玉莲
黄桦瑞
张秀秀
朱红惠
YANG Fan;SU Boli;WANG Yonghong;ZHANG Yulian;HUANG Huarui;ZHANG Xiuxiu;ZHU Honghui(Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,State Key Laboratory of Applied Microbiology Southern China,Guangdong Microbial Culture Collection Center(GDMCC),Guangdong Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510070,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第17期27-32,共6页
Food and Fermentation Industries
基金
广东省科技计划项目(2016A010105013,2019B030316017)
广东省重点领域研发计划(2018B020206001)
广州市产学研协同创新重大专项(201806010065)。
关键词
代谢工程
大肠杆菌
启动子
番茄红素
metabolic engineering
Escherichia coli
promoters
lycopene
