摘要
目的:建立超高效液相色谱-串联质谱(ultra performance liquid chromatography mass spectrometry/mass spectrometry,UPLC-MS/MS)法同时测定参蛤胶囊中人参皂苷Rb1、Rg1、Re和三七皂苷R1含量的方法。方法:采用ACQUITY UPLC BEN C18色谱柱(2.1 mm×50.0 mm,1.7μm),以乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,流速为0.3 mL·min^-1,柱温为30℃;采用电喷雾离子源(ESI),正离子检测,多反应监测模式。结果:人参皂苷Rb1、Rg1、Re和三七皂苷R1的线性范围分别为1.018~20.356 mg·L^-1(r=0.9994)、1.027~20.550 mg·L^-1(r=0.9996)、1.041~20.828 mg·L^-1(r=0.9995)、0.923~18.468 mg·L^-1(r=0.9996),线性关系良好,平均加样回收率(n=6)为94.15%~96.18%,RSD为2.67%~3.00%。结论:UPLC-MS/MS法快速、准确、灵敏度高,可用于参蛤胶囊的质量评价及控制。
Objective:To establish an ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method for the simultaneous determination of ginsenoside Rb1,Rg1,Re and notoginsenoside R1 in Ginseng and Gecko Capsule.Methods:ACQUITY UPLC BEN C18 column(2.1 mm 50.0 mm,1.7 m)was used.The mobile phase was acetonitrile-0.1%formic acid solution.The gradient elution was carried out at a flow rate of 0.3 mL·min-1 and with a column temperature of 30 degrees.Electrospray ionization(ESI),positive ion detection and multi reaction monitoring mode were used.Results:The linear ranges of ginsenoside Rb1,Rg1,Re and notoginsenoside R1 were1.018-20.356 mg·L^-1(r=0.9994),1.027-20.550 mg·L^-1(r=0.9996),1.041-20.828 mg·L^-1(r=0.9995),0.923-18.468 mg·L^-1(r=0.9996)respectively.The linear relationship was good,and the average sample recovery(n=6)was 94.15%-96.18%,and RSD was 2.67%-3.49%.Conclusion:UPLC-MS/MS method is rapid,accurate and sensitive,and can be used for quality evaluation and control of Ginseng and Gecko Capsule.
作者
张丹
岳磊
李凯
ZHANG Dan;Yue Lei;LI Kai(Zhengzhou Institute of Food and Drug Control,Zhengzhou,Henan,China,450006;Henan University of CM,Henan Provincial Center for Processing Technology of TCM,Zhengzhou,Henan,China,450046)
出处
《河南中医》
2020年第9期1438-1442,共5页
Henan Traditional Chinese Medicine
基金
河南省高等学校青年骨干教师培养计划项目(2018GGJS083)。
