摘要
研究了藻-菌混合培养(M)、添加NaHCO3培养(C)以及二者的结合培养(MC)对栅藻生长和脂类合成的影响。将从栅藻培养液中分离的4种微生物与栅藻进行混合培养,筛选出对栅藻生长促进效果最佳的细菌B1,并确定栅藻与B1的最佳接种比例;确定添加NaHCO3培养的最佳NaHCO3质量浓度;按照确定的最佳参数进行M、C和MC实验,测定每种培养方式的生长指标和脂质合成指标,并与栅藻单独培养进行对比分析。结果表明:与栅藻单独培养相比,M、C和MC的生物量分别提高了68.3%、98.8%、195.1%,脂类含量提高35.6%、41.6%、50.4%,脂肪酸组成中的C18∶1总含量提高了17.0%、30.9%、47.4%。因此,MC比M和C更高效,是一种有潜在应用价值的生物柴油原料生产模式。
The effects of algae-bacteria mixed culture(M), supplement of NaHCO3 culture(C), and the combination of both(MC) on the growth and lipid synthesis of Scenedesmus obliquus were studied. Four microorganisms separated from algae broth were mixed cultured with S. obliquus,then the best bacterial B1 for promoting growth of S. obliquus was selected and the optimal inoculation ratio was determined;the optimal NaHCO3mass concentration in supplement of NaHCO3 culture was determined;then the M, C and MC experiments were carried out,the growth indexes and lipid synthesis indexes of the culture methods were determined and compared with individual culture. The results showed that compared with the individual culture, the biomasses and lipid contents of S. obliquus by M, C and MC increased by 68.3%, 98.8%, 195.1% and 35.6%, 41.6%, 50.4%, respectively;and the C18∶1 contents in the lipid increased by 17.0%, 30.9% and 47.4%, respectively. Therefore, MC was more efficient than M and C, and MC had potential application value as a biodiesel raw material production mode.
作者
王雪晴
邢向英
董庆霖
燕然
李文娜
WANG Xueqing;XING Xiangying;DONG Qinglin;YAN Ran;LI Wenna(College of Chemical Engineering,Hebei University of Technology,Tianjin 300130,China;Metabolic Engineering Laboratory,Hebei University of Technology,Tianjin 300130,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2020年第8期96-102,共7页
China Oils and Fats
基金
河北省自然科学基金项目(B2008000029)。
关键词
藻-菌混合培养
栅藻
生长
脂类合成
生物柴油
algae-bacteria mixed culture
Scene-desmus obliquus
growth
lipid synthesis
biodiesel
