摘要
为得到较佳植物抑菌物质的配方比,以丁香、肉桂等7种植物为试材,采用响应面法对植物提取物及其复配物进行抑菌(葡萄座腔菌)活性分析。试验结果表明:肉桂、丁香、黄芩、菖蒲等7种植物提取物抑菌效果均较好,半抑制率IC_(50)在0.91~1.41 mg/mL,在处理浓度为0.1~1.5 mg/mL时,各提取物对菌丝生长抑制率均与浓度呈正比关系。黄芩、丁香、肉桂、皂角刺、广藿香、菖蒲、油茶粕的最佳配方比为1.375∶1.125∶0.45∶0.5∶1.35∶1.25∶2.8,此配方下所得复配物的IC_(50)为0.489 mg/mL,当浓度达到0.8 mg/mL时,菌丝生长抑制率达85%以上。该复配物抑菌效果好且经济实惠,为今后绿色、有机果蔬保鲜剂的开发与利用奠定一定的理论基础。
In order to obtain better formula of plant antimicrobial substances,seven species of medical plants,such as clove and cinnamon were used as test materials to analyze the antimicrobial(Botryosphaeria dothidea)activity of the plant extracts and their compounds by Response Surface Methodology(RSM).The results showed that all seven extracts of cinnamon,clove,scutellaria baicalensis and acorus calamus etc.had better bacteriostasis effcet.The semi-inhibition rate(IC50)of the extracts was 0.91~1.41 mg/mL.At the concentration of 0.1~1.5 mg/mL,the inhibition rate of the extract on mycelial growth was proportional to its concentration.The best ratio of scutellaria,clove,cinnamon,spina gleditsiae,patchouli,calamus and camellia seed meal was 1.375∶1.125∶0.45∶0.5∶1.35∶1.25∶2.8,respectively.The IC50 of the compound prepared with above formula was 0.489 mg/mL.When the concentration reached 0.8 mg/mL,the inhibitory rate of mycelial growth was over 85%.The compound has good bacteriostatic effect and economical benefit.Which lays a theoretical foundation for the development and utilization of green and organic preservatives for fruits and vegetables in the future.
作者
石浩
王仁才
王芳芳
王琰
卜范文
周倩
SHI Hao;WANG Ren-cai;WANG Fang-fang;WANG Yan;BU Fan-wen;ZHOU Qian(College of Horticulture,Hunan Agricultural University,Changsha 410128,China;College of Agriculture and Forestry Science,Hunan Applied Technology University,Changde 415100,China;Research Institute of Horticulture in Hunan Province,Changsha 410125,China)
出处
《保鲜与加工》
CAS
北大核心
2020年第5期27-33,共7页
Storage and Process
基金
湖南省科技计划项目(2017NK2071
2018NK2013)
湖南省教育厅科学研究项目(19C1353)。
关键词
响应面法
植物提取物
猕猴桃
采后
葡萄座腔菌
协同抑菌
response surface methodology
plant extract
kiwifruit
postharvest
Botryosphaeria dothidea
synergistic bacteriostasis
