摘要
茉莉酸甲酯(Methyl jasmonate,MeJA)与植物间的防御通讯密切相关,其挥发性强,可以在空气中传播而诱导邻近植物产生防御反应,为探明MeJA诱导邻近植株抗虫性的浓度和距离效应,本研究在大棚(长4 m×宽1 m×高1 m)进口处喷以不同浓度MeJA,检测离气味源60 cm、120 cm、180 cm、240 cm和300 cm远的番茄植株叶片多酚氧化酶(Polyphenol oxidase,PPO),过氧化物酶(Peroxidase,POD)和脂氧合酶(Lipoxygenase,LOX)的活性以及斜纹夜蛾幼虫取食不同处理番茄植株叶片后的体重增长率,研究结果表明1 mmol/L和10 mmol/L MeJA挥发物可以显著诱导增强邻近番茄植株叶片PPO,POD和LOX酶的活性,以离10 mmol/L MeJA气味源60 cm处植株叶片的酶活性最高,并随着距离的增加而呈降低的趋势;取食60 cm和300 cm对照组植株叶片的斜纹夜蛾幼虫其体重增长率没有显著差异,然而1 mmol/L和10 mmol/L MeJA处理下,取食300 cm处植株叶片的斜纹夜蛾幼虫其体重增长率显著高于取食60 cm处的,相同时间下以取食10 mmol/L MeJA处理组60 cm处植株叶片的幼虫体重增长率最低,以上研究结果表明MeJA对邻近番茄植株抗虫性的诱导有明显的浓度和距离效应。
Methyl jasmonate(MeJA)is closely related to interplant communication.It can act as a volatile signal to travel through the atmosphere to induce plant defense.In order to explore the concentration and distance effects of MeJA volatile on neighboring plants′resistance against herbivore insects,we placed tomato plants at distance of 60 cm,120 cm,180 cm,240 cm and 300 cm from the entrance,and then sprayed MeJA at the entrance of the greenhouse(length 4 m×width 1 m×height 1 m),activities of peroxidase(PPO),polyphenol oxidase(POD)and lipoxygenase(LOX)of leaves of tomato plants at different distance away from MeJA odour as well as Spodopter litura larvae mass gain fed on plants at distance of 60 cm and 300 cm were tested.Our results showed that airborne MeJA volatilized from 1 mmol/L and 10 mmol/L MeJA could significantly induce PPO,POD and LOX activities in the leaves of tomato plants,and the induced effects were most prominent on tomato plants placed at the distance of 60 cm from 10 mmol/L MeJA volatile,but the inductive effects decreased with the increasing distance.S.litura larvae fed on plants placed at distance of 300 cm from 1 mmol/L and 10 mmol/L MeJA volatile gained more mass than those fed on plants placed at distance of 60 cm,while mass of larvae fed on 10 mmol/L MeJA treated group were the least at the same feeding time.Collectively,these results indicate that volatile MeJA resulted in significant concentration and distance effects on tomato plants′resistance against S.litura.
作者
宋佳
王骏
叶茂
SONG Jia;WANG Jun;YE Mao(Institute of Entomology,Guizhou University,Guiyang 550025,China;Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Regions,Guiyang 550025,China)
出处
《生态学报》
CAS
CSCD
北大核心
2020年第15期5433-5440,共8页
Acta Ecologica Sinica
基金
国家自然科学基金项目(31701801)
贵州省科技计划项目(黔科合基础[2016]1043,黔科合LH字[2017]7276)
贵州大学引进人才项目([2016]43)。
关键词
茉莉酸甲酯挥发物
邻近植株抗虫性
浓度和距离效应
methyl jasmonate volatile
resistance of neighboring plants
concentration and distance effects
