摘要
本文旨在建立一种乳饮料中异构化乳糖的酶法测定方法。乳饮料样品经过亚铁氰化钾和硫酸锌沉淀蛋白质、β-D-半乳糖苷酶水解乳糖和异构化乳糖、葡萄糖氧化酶氧化葡萄糖等处理过程,用紫外分光光度计检测乳饮料中异构化乳糖的含量。该方法具有良好的回收率,为83.5%~92.7%,以及良好的重复性,相对标准偏差(RSD)为4.04%~6.05%(n=6)。该方法检测结果准确且稳定性好,适用于乳饮料中异构化乳糖的检测。
An enzymatic method for the determination of lactulose in milk beverages was established.The milk beverages samples were treated with potassium ferrocyanide and zinc sulfate to precipitate protein,β-D-galactosidase to hydrolyze lactose and lactulose,and glucose oxidase to oxidize glucose,the content of lactulose in milk beverage was determined by UV spectrophotometer.The method has a good recovery rate of 83.5%~92.7%,and a good repeatability,the relative standard deviation(RSD)is 4.04%~6.05%(n=6).The method has high sensitivity,good stability and accurate detection results,and is suitable for the detection of lactulose in milk beverages.
作者
付云双
赵贞
万鹏
温国艳
黄文强
徐红
李翠枝
吕志勇
FU Yun-shuang;ZHAO Zhen;WAN Peng;WEN Guo-yan;HUANG Wen-qiang;XU Hong;LI Cui-zhi;LV Zhi-yong(Inner Mongolia Yili Industrial Group Co.,Ltd.Hohhot 010110,China)
出处
《饮料工业》
2020年第4期35-38,共4页
Beverage Industry
关键词
酶法
异构化乳糖
乳饮料
enzymatic method
lactulose
milk beverage
