摘要
目的研究临床分离出的耐碳青霉烯类肺炎克雷伯菌(CRKP)对84消毒液的抗性,为做好医院环境消毒提供参考。方法通过基因测定和最小杀菌浓度测定方法,对某医院临床分离的CRKP抗消毒剂基因携带情况和84消毒液抗性进行观察。结果从临床分离的77株CRKP检出84消毒液抗性株为8株,阳性率为10. 4%;从70株普通肺炎克雷伯菌中检出84消毒液抗性株为3株,阳性率为4. 3%。84消毒液抗性菌株中抗消毒剂基因qacEΔ1-sul1阳性率为54. 5%,非抗性菌株中该基因阳性率为10. 5%。结论部分CRKP已对84消毒液产生了轻度的抗性。抗消毒剂基因qacEΔ1-sul1与84消毒液的抗性之间存在一定的相关性。
Objective To study the resistance of clinically isolated carbapenem-resistant Klebsiella pneumoniae( CRKP)to 84 disinfectant,and to provide a reference for disinfection of hospital environment. Methods Through gene detection and minimum bactericidal concentration determination methods,the anti-disinfectant genes carrying and 84 disinfectant resistance of clinically isolated CRKP in a hospital were observed. Results 8 strains of 84 disinfectant-resistant bacteria were detected from 77 strains of clinically isolated CRKP,with a positive rate of 10. 4%;3 strains of 84 disinfectant-resistant bacteria were detected from 70 strains of common Klebsiella pneumoniae,with a positive rate of 4. 3%. The positive rate of the anti-disinfectant gene qacEΔ1-sul1 in 84 disinfectant-resistant strains was 54. 5%,and the positive rate of the gene in non-resistant strains was 10. 5%. Conclusion Some strains of CRKP have developed mild resistance to 84 disinfectant. There is a certain correlation between the anti-disinfectant gene qacEΔ1-sul1 and the 84 disinfectant resistance.
作者
李祥
鲁辛辛
LI Xiang;LU Xin—xin(Bejing Tongren Hospital,Capital Medical University,Bejing 100730;Bejing Chuiyangliu Hospital,China)
出处
《中国消毒学杂志》
CAS
2020年第8期564-566,共3页
Chinese Journal of Disinfection
关键词
耐碳青霉烯类肺炎克雷伯菌
抗消毒剂基因
84消毒液
抗性
Carbapenem-resistant Klebsiella pneumoniae
anti-disinfectant gene
84 disinfectant
resistance
