摘要
目的对一起外籍船舶船员感染性腹泻疫情进行病原学检测。方法采集外籍船舶船员的粪便10份、肛拭子样本16份、血液9份、其他样品6份。依照《感染性腹泻诊断标准》(WS 271—2007)和《预防医学微生物学及检验技术》,通过细菌培养、核酸检测及毒力基因检测等方法来鉴定本起感染性腹泻疫情是否由O157∶H7引起。结果从腹泻患者的肛拭子和粪便中,检出2份肠出血性大肠杆菌EHEC-stx1基因核酸阳性,患者血清中检出志贺毒素EHECstx1,并且从肛拭子和粪便中培养出2株肠出血性大肠杆菌菌株。结论本次外籍船舶船员感染性腹泻是由肠出血性大肠杆菌引起,并排除本次感染性腹泻是由O157∶H7引起的疫情。
Objective To carry out the pathogenic detection of an infectious diarrhea in foreign ship crew.Methods A total of 10 feces,16 anal swabs,9 blood samples and 6 other samples were collected from foreign ship crew.According to the Diagnostic Criteria for Infectious Diarrhea(WS 271-2007)and Microbiology and Testing Techniques of Preventive Medicine,whether the epidemic of infectious diarrhea was caused by O157∶H7 was identified by bacterial culture,nucleic acid detection and virulence gene detection.Results Two cases of EHEC-stx1 gene’s nucleic acid were detected from anal swab and faeces of diarrhea patients,and Shigoxin EHEC-stx1 was detected in the serum of the patients,and two strains of Escherichia coli were cultured from the anal swab and faeces.Conclusion The infectious diarrhea in foreign ship crew was caused by enterohemorrhagic Escherichia coli,and the infection caused by O157∶H7 were excluded.
作者
吴巨飞
王海燕
WU Ju-fei;WANG Hai-yan(Yancheng Municipal Center for Disease Control and Prevention,Yancheng,Jiangsu 224002,China)
出处
《中国卫生检验杂志》
CAS
2020年第16期1967-1969,共3页
Chinese Journal of Health Laboratory Technology
