摘要
以南极磷虾肽(antarctic krill peptides,AKP)和FeCl2·4H2O为原料,以亚铁螯合率和螯合物得率为评价指标,在单因素试验基础上,利用正交试验优化制备出南极磷虾肽-亚铁螯合物(antarctic krill peptide-ferrous chelates,AKP-Fe),并对其理化性质进行分析。结果表明:AKP-Fe的最佳制备条件为:肽铁质量比3∶1,螯合温度40℃,螯合时间40 min,pH值5.5,肽浓度4%,乙醇体积倍数6。在此条件下测得亚铁螯合率为77.25%,螯合物得率为41.03%。傅里叶变换红外光谱分析显示AKP的氨基和羧基与Fe2+发生结合;扫描电镜分析显示AKP与Fe2+螯合后由松疏的片状结构变为颗粒状的聚集体。
Antarctic krill peptides(AKP)and FeCl2·4H2O were used as the raw material.The effects of various factors on iron-chelating rate and chelate yield were investigated and the chelating process were optimized.Using one-factor-at-a-time method and orthogonal test,and the characterization of peptides-ferrous chelate was investigated.The optimal chelating conditions were obtained as follows that AKP-Fe dichloride mass ratio of 3∶1,chelating temperature of 40℃,chelating time of 40 min,pH value of 5.5,AKP concentration of 4%,ethanol volume ratio of 6.The actual iron-chelating rate under the optimized condition was 77.25%,chelate yield was 41.03%.Fourier transform infrared spectroscopy(FTIR)analysis suggested that the amino and carboxyl groups of AKP were chelated with Fe2+.Scanning electron microscopy(SEM)images showed that the microstructure of AKP changed dramatically after the chelation with Fe2+,turning from loose and sparse flake structure into granular aggregates.
作者
林海燕
王珊珊
马玉洁
吕世伟
周德庆
LIN Hai-yan;WANG Shan-shan;MA Yu-jie;Lü Shi-wei;ZHOU De-qing(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Laboratory for Marine Drugs and Bioproducts,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266071,Shandong,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)
出处
《食品研究与开发》
CAS
北大核心
2020年第19期87-93,共7页
Food Research and Development
基金
国家自然科学基金青年科学基金项目(41706166)。
关键词
南极磷虾肽
肽-亚铁螯合物
制备
亚铁螯合率
理化性质
antarctic krill peptides
peptide-ferrous chelate
preparation
iron-chelating rate
physicochemical properties
