摘要
目的:探讨miR-29b-2基因单核苷酸位点rs1474742A/C和rs12401619C/G多态性在我国广西人群的分布情况,比较其与其他人群的多态性差异,并统计分析不同基因型的常见血脂检测项目水平。方法:SNPscan法对291例研究对象rs1474742A/C和rs12401619C/G位点进行分型,SPSS23.0软件统计分析。结果:rs1474742A/C具有AA、AC和CC基因型,广西人群rs1474742A/C基因型和等位基因频率与千人基因组计划公布的美国人、欧洲人、日本人、意大利人和中国汉族人的差异均有统计学意义(P<0.01)。rs12401619C/G具有CC、CG和GG基因型,基因型频率和等位基因频率与美国人、欧洲人、日本人、意大利人和中国汉族人相比差异均有统计学意义(P<0.01)。rs1474742A/C位点AC/AA和CC在LDL-C中差异具有统计学意义(P<0.05)。结论:miR-29b-2基因rs1474742A/C和rs12401619C/G位点的多态性在不同人群中差异具有统计学意义。rs1474742A/C基因型与LDL-C水平相关。
Objective:To explore distribution situation of miR-29b-2 gene single-nucleotide sites rs1474742A/C and rs12401619C/G polymorphisms in Guangxi population and compare its distribution differences with other populations.To analyze level of common blood lipid indexes in genotypes.Methods:SNPscan to test genotypes of rs1474742A/C and rs12401619C/G in 291 Guangxi population.SPSS23.0 software for statistical analysis.Results:rs1474742A/C has genotypes of AA,AC and CC,whose statistical difference was shown in distribution frequencies of genotypes and alleles of between Guangxi population and American,European,Japanese,Italian and Chinese Han population from 1000 Genomes(P<0.01).rs12401619C/G has genotypes of AA,AG and GG,who has significant difference of distribution frequencies of genotypes and alleles between Guangxi population American,European,Japanese,Italian and Chinese Han population(P<0.01).Level of LDC-C was different in AC/AA and CC of rs1474742A/C(P<0.05).Conclusion:Polymorphisms of rs1474742A/C and rs12401619C/G in miR-29b-2 gene present difference in various population.rs1474742A/C polymorphism also associated with LDL-C level.
作者
何永玲
王彩梅
杨峻
曾志能
乔永超
韦叶生(指导)
HE Yong-Ling;WANG Cai-Mei;YANG Jun;ZENG Zhi-Neng;QIAO Yong-Chao;WEI Ye-Sheng(Department of Laboratory Medicine,Affiliated Hospital of Guilin Medical University,Guilin 541000,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2020年第16期1994-1997,共4页
Chinese Journal of Immunology
基金
国家自然科学基金(81560552)
广西重点研发课题(2018AB58018)
广西自然科学基金(2018GXNSFAA138120)
桂林市科学研究与技术开发计划项目(No.20190218-4-1)资助。
