摘要
目的建立UPLC-FLD法同时测定白芷中水合氧化前胡素、佛手柑内酯、氧化前胡素、欧前胡素、异欧前胡素的含有量。方法白芷甲醇提取物的分析采用Agilent ZORBAX ECLIPSE Plus C18柱(2.1 mm×100 mm,1.8μm);流动相0.1%甲酸水-乙腈;体积流量0.4 mL/min;柱温35℃;荧光检测器激发波长259 nm,发射波长490 nm。结果5种成分在各自范围内线性关系良好(r=0.9999),平均加样回收率98.7%~102.5%,RSD 1.02%~4.17%。结论该方法准确稳定,重复性好,可用于白芷的质量控制。
AIM To establish a UPLC-FLD method for the simultaneous determination of oxypeucedanin hydrate,bergapten,oxypeucedanin,imperatorin and isoimperatorin in Angelicae Dahuricae Radix.METHODS The analysis of methanol extract of A.Dahuricae was performed on a 35℃thermostatic Agilent ZORBAX ECLIPSE Plus C18 column(2.1 mm×100 mm,1.8μm),with the mobile phase comprising of 0.1%formic acid water-acetonitrile flowing at 0.4 mL/min in a gradient elution manner,and the excitation wavelength and emission wavelength were set at 259 nm and 490 nm,respectively.RESULTS Five constituents showed good linear relationships within their own ranges(r=0.9999),whose average recoveries were 98.7%-102.5%with the RSDs of 1.02%-4.17%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of A.dahuricae.
作者
李乾
刘洁
李月婷
阳娇
赵淑军
肖红斌
LI Qian;LIU Jie;LI Yue-ting;YANG Jiao;ZHAO Shu-jun;XIAO Hong-bin(School of Pharmacy,Shihezi University,Shihezi 832002,China;Research Center for Chinese Medicine Analysis and Transformation,Beijing University of Chinese Medicine,Beijing 100029,China;Beijing Research Institute of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;School of Chinese Materia Medical,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《中成药》
CAS
CSCD
北大核心
2020年第9期2357-2362,共6页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(81774155,81803703)。
