摘要
该文建立了基于表面增强拉曼光谱(SERS)技术检测水和尿液中舒芬太尼的方法。首先制备了银溶胶SERS基底并进行表征。随后通过理论计算和实验对比,对舒芬太尼的拉曼特征峰进行了归属。通过探索最佳实验条件,确定了促凝剂及其浓度。对水和人工尿液中的舒芬太尼进行了SERS检测,检出限分别为0.09μg/mL和1.55μg/mL。水和尿液中舒芬太尼的特征峰(1004 cm^-1)强度与舒芬太尼质量浓度在一定范围内呈线性关系,相关系数(r^2)分别为0.979和0.968。水和尿液中舒芬太尼的回收率分别为96.3%~107%和95.8%~106%,相对标准偏差分别为4.2%~4.7%和3.3%~5.2%。该方法具有快速、准确、无损、操作简便等优点,为水和尿液中舒芬太尼的快速检测打下了良好的基础。
A method based on surface enhanced Raman spectroscopy(SERS)was established for the detection of sufentanil in water and urine in this paper.Meanwhile,a silver colloid substrate for SERS was prepared and characterized,and the Raman characteristic peaks of sufentanil were assigned by theoretical calculation and experimental comparison.The optimum experimental conditions were investigated,and the coagulant and its concentration were determined.SERS detection on sufentanil in water and artificial urine was performed,with the detection limits of 0.09μg/mL and 1.55μg/mL,respectively.There existed good linear relationships for the characteristic peak(1004 cm^-1)intensities of sufentanil in water and urine in a certain mass concentration range,with their correlation coefficient(r^2)of 0.979 and 0.968,respectively.The recoveries for the analytes were in the ranges of 96.3%-107%and 95.8%-106%,with their relative standard deviations of 4.2%-4.7%and 3.3%-5.2%,respectively.The developed method is rapid,accurate,non-destructive and user-friendly,and it lays a good foundation for the rapid detection of sufentanil in water and urine.
作者
陈志杰
马小卫
陈薪璇
韩斯琴高娃
哈斯乌力吉
CHEN Zhi-jie;MA Xiao-wei;CHEN Xin-xuan;HAN Si-qin-gao-wa;HASI Wu-li-ji(Department of Ophthalmology,Harbin 242 Hospital,Harbin 150066,China;Affiliated Hospital of Inner Mongolia University for the Nationalities,Tongliao 028007,China;National Key Laboratory of Science and Technology on Tunable Laser,Harbin Institute of Technology,Harbin 150080,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2020年第9期1073-1078,1084,共7页
Journal of Instrumental Analysis
基金
国家自然科学基金(31871873)
内蒙古自治区自然科学基金(2018LH08055)。
