摘要
目的探讨结肠癌转移相关基因1(MACC1)在侵袭转移中的作用及其机制。方法收集2016年7月至2017年9月于江南大学附属医院手术切除肺癌组织及其对应的癌旁组织180例,构建组织微阵列免疫组织化学检测组织中MACC1与β-连环蛋白(β-catenin)的表达情况。采用慢病毒介导的小干扰RNA(siRNA)下调MACC1在A549细胞中的表达,分为对照组、慢病毒空载体组和敲低组。构建短发卡RNA(shRNA)质粒实现MACC1-shRNA干扰质粒后慢病毒感染H1299细胞(购自美国ATCC公司)使MACC1基因的沉默,分为质粒转染组与慢病毒空载体组,采用5-乙炔基-2’脱氧尿嘧啶核苷(EdU)法、Transwell小室实验、划痕试验分别检测细胞增殖能力、侵袭性和迁移性。组间比较采用两独立样本t检验。结果组织芯片免疫组织化学显示肺腺癌组织中MACC1与β-catenin的表达水平呈正相关(相关系数0.397,P<0.05);慢病毒介导A549细胞MACC1下调后引起β-catenin表达下降[对照组(0.83±0.04)比空载体组(0.91±0.09,t=-0.241,P>0.05;空载体组(0.91±0.09)比敲低组(0.24±0.02,t=1.940,P<0.05);对照组(0.83±0.04)比敲低组(0.24±0.02,t=1.699,P<0.05];沉默MACC1后行反转录-聚合酶链反应(RT-PCR)检测H1299的β-catenin表达显著下降(0.79±0.07比0.38±0.08,t=3.267,P<0.05),且质粒转染组H1299细胞平均穿膜细胞数[(18.3±2.36)个]少于慢病毒空载体组[(36.2±2.1)个,t=-13.460,P<0.05];划痕实验显示24 h后质粒转染组[(266±27)μm]距离明显小于慢病毒空载体组[(931±36)μm,t=2.631,P<0.01]。结论MACC1在肺腺癌中高表达,促进癌基因β-catenin的表达,从而促进肺腺癌的侵袭转移。
Objective:To investigate the role and mechanism of colon cancer metastatic gene 1(MACC1)in the invasion and metastasis of lung adenocarcinoma.Methods:From July 2016 to September 2017,180 cases of lung cancer and their corresponding paracancerous tissues were surgically removed in the Affiliated Hospital of Jiangnan University.Tissue microarray immunohistochemistry was used to detect the expression of MACC1 andβ-catenin.Lentivirus-mediated small interfering RNA(siRNA)was used to down-regulate the expression of MACC1 in A549 cells,and the cells were divided into control group,lentivirus empty vector group and knock-down group.Short hairpin RNA(shRNA)plasmid was constructed to silence MACC1 gene in H1299 cells infected with lentivirus after MACC1-shRNA interference plasmid.EdU,Transwell test and scratch test were used to detect cell proliferation,invasiveness and migration,respectively.Two independent samples t-test was used to analyze the statistical significance.Results:Tissue microarray immunohistochemistry showed that there was a positive correlation between the expression of MACC1 andβ-catenin in lung adenocarcinoma(correlation coefficient 0.397,P<0.05).The expression ofβ-catenin decreased after down-regulation of lentivirus-mediated MACC1 in A549 cells(Control:0.83±0.04 vs.NC:0.91±0.09,t=-0.241,P>0.05;NC:0.91±0.09 vs.KD:0.24±0.02,t=1.940,P<0.05;Control:0.83±0.04 vs.KD:0.24±0.02,t=1.699,P<0.05).After silencing MACC1,the expression ofβ-catenin in H1299 was significantly decreased(0.79±0.07 vs.0.38±0.08,t=3.267,P<0.05),and the average number of transmembrane cells in H1299(18.3±2.36)was significantly lower than that in lentivirus empty vector group(36.2±2.1,t=-13.460,P<0.05).Scratch test showed that the distance of plasmid transfection group[(266±27)μm]was significantly lower than that of lentivirus empty vector group[(931±36)μm,t=2.631,P<0.01]after 24 h.Conclusion:MACC1 is highly expressed in lung adenocarcinoma,which promotes the expression of oncogeneβ-catenin,thus promoting the invasion and metastasis of lung adenocarcinoma.
作者
金思豪
庞庆丰
秦晓云
刘钢
王志强
Jin Sihao;Pang Qingfeng;Qin Xiaoyun;Liu Gang;Wang Zhiqiang(Department of Thoracic and Cardiovascular Surgery,Affiliated Hospital of Jiangnan University,Wuxi 214062,China;Wuxi Medical College,Jiangnan University,Wuxi 214122,China;Department of Operating Room,Affiliated Hospital of Jiangnan University,Wuxi 214062,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2020年第7期1325-1327,共3页
Chinese Journal of Experimental Surgery
基金
江苏省青年医学重点人才项目(QNRC2016156)
江苏省自然科学基金面上项目(BK20161141)。
