摘要
目的建立一种超高效液相色谱(UHPLC-UV)波长切换法同时测定风寒感冒颗粒中D-苦杏仁苷、葛根素、橙皮苷等14种化学成分的含量,结合化学计量学分析不同来源样品的质量差异。方法色谱柱为AgilentPoroshell120EC-C18(150 mm×2.1 mm,2.7μm),以乙腈-0.1%磷酸水溶液为流动相梯度洗脱,切换波长(210、254、310 nm)对5家生产企业68批样品中14种化学成分含量进行测定;并使用雷达图分析、相似度评价、热图聚类分析和主成分分析法对数据进行统计分析。结果 68批样品中各成分质量分数分别为D-苦杏仁苷0.063~3.885 mg/g、3′-羟基葛根素0.012~1.540 mg/g、葛根素0.036~4.017 mg/g、3′-甲氧基葛根素0.016~1.837 mg/g、葛根素-6″-O-木糖苷0.004~0.449 mg/g、芹糖葛根素苷0.021~2.076mg/g、大豆苷0.010~1.527 mg/g、升麻素苷0.007~0.471 mg/g、5-O-甲基维斯阿米醇苷0.062~1.029 mg/g、橙皮苷0.210~8.453 mg/g、迷迭香酸0.001~0.237 mg/g、水合氧化前胡素0.007~0.204 mg/g、甘草酸0.056~1.311 mg/g、氧化前胡素0.002~0.042 mg/g。化学计量学分析表明,同一厂家不同批次样品的整体质量一致性较好,但不同厂家之间样品的整体质量一致性存在较大差异。结论建立的基于UHPLC波长切换法的多组分含量测定方法简便、重复性好、专属性强,可为风寒感冒颗粒的整体质量评控提供方法参考。
Objective To establish a method for the simultaneous content determination of 14 chemical components such as Damygdalin, puerarin, hesperidin in Fenghan Ganmao Granules(FGG) by UHPLC-UV wavelength switching method, and chemometric analysis was used to analyze the quality differences. Methods Separation was performed on an Agilent Poroshell 120 EC-C18(150 mm × 2.1 mm, 2.7 μm) with a gradient elution of acetonitrile and 0.1% phosphoric acid. The content of 14 chemical components in 68 batches of samples from five manufacturers was determined by switching wavelength(210, 254, 310 nm). The radar chart, similarity evaluation, heat map and hierarchical clustering analysis, and principal component analysis(PCA) were used for data analysis. Results The content of each component was as follows, D-amygdalin 0.063—3.885 mg/g, 3′-hydroxy puerarin 0.012—1.540 mg/g, puerarin 0.036—4.017 mg/g, 3′-methoxy puerarin 0.016—1.837 mg/g, puerarin-6″-O-xyloside 0.004—0.449 mg/g, mirificin 0.021—2.076 mg/g, daidzin 0.010—1.527 mg/g, prim-O-glucosylcimifugin 0.007—0.471 mg/g, 5-O-methylvisammioside 0.062—1.029 mg/g, hesperidin 0.210—8.453 mg/g, rosmarinic acid 0.001—0.237 mg/g, oxypeucedanin hydrate 0.007—0.204 mg/g, glycyrrhizic acid 0.056—1.311 mg/g, oxypeucedanin 0.002—0.042 mg/g, respectively. Chemometric analysis showed that there were some differences among the samples from different manufacturers, and the samples from the same manufacturer were more consistent. Conclusion The method is simple, reproducible, and specific, which provides a reference method for the overall quality evaluation of FGG.
作者
许莉
黄晓婧
高鹏
赵小勤
文永盛
谭鹏
XU Li;HUANG Xiao-jing;GAO Peng;ZHAO Xiao-qin;WEN Yong-sheng;TAN Peng(Chengdu Institute for Food and Drug Control,NMPA Key Laboratory for Quality Monitoring and Evaluation of Traditional Chinese Medicine,Chengdu 610045,China;Sichuan Academy of Traditional Chinese Medicine,State Key Laboratory of Quality Evaluation of Traditional Chinese Medicine,Chengdu 610041,China)
出处
《中草药》
CAS
CSCD
北大核心
2020年第14期3670-3678,共9页
Chinese Traditional and Herbal Drugs
基金
四川省科技厅应用基础研究项目(2019YJ0640)
四川省重大科技专项(2018TZDZX0007)。
