摘要
目的分析不同产地不同品系瓜蒌皮中多类型资源性成分差异,为其资源化利用提供数据支撑。方法采用UPLC-MS/MS方法对8个产地9个品系22个批次瓜蒌皮中的糖类、核苷类、氨基酸类、黄酮类、三萜类等39种化学成分含量进行分析,利用主成分分析(PCA)法对瓜蒌皮产地及品系差异进行综合评价。结果瓜蒌皮中果糖、葡萄糖的平均含量分别为14.78%、8.14%;检测出10种核苷类、18种游离氨基酸类成分,核苷类成分平均总量为0.57 mg/g,游离氨基酸类成分平均总量为37.71 mg/g;检测出黄酮类成分6种、三萜类成分3种,平均总量分别为0.58、0.01 mg/g。PCA结果表明,基于39种化学成分分析结果,不同产地不同品系瓜蒌皮品质存在明显差异。河北石家庄产“皖蒌7号”瓜蒌皮综合得分最高,河北产区与其他产区的瓜蒌皮差异明显,主要差异成分为药效成分氨基酸类和木犀草苷。结论不同产地不同品系瓜蒌皮所含资源性成分种类基本一致,但从整体上分析评价,各类型资源性成分含量差异较大,传统药用瓜蒌产区所产部分品系瓜蒌皮中资源性成分总量及药效成分含量相比其他产区较高,研究结果可为瓜蒌皮临床用药选择和不同产地不同品系瓜蒌皮的综合利用提供参考。
OBJECTIVE To analyze the multiple types of chemical constituents in the Trichosanthis Pericarpium from different producing areas and strains,then to provide the data support for their resource utilization.METHODS Twenty-two batches of Trichosanthis Pericarpium were collected from eight different areas,with nine strains.UPLC-MS/MS method was employed to simultaneously determine the contents of thirty-nine chemical components,including sugars,nucleosides,amino acids,flavonoids,triterpenes,and so on.Principal component analysis(PCA)were applied to comprehensively evaluate the differences in producing areas and strains of Trichosanthis Pericarpium.RESULTS The average contents of fructose and glucose in Trichosanthis Pericarpium were 14.78%and 8.14%,respectively.Ten nucleosides and eighteen kinds of free amino acids were detected.The average total amounts of nucleosides and free amino acids were 0.57 mg/g and 37.71 mg/g,respectively.Six flavonoids and three triterpenoids were detected,and the average total amounts were 0.58 mg/g and 0.01 mg/g,respectively.PCA showed that,based on the 39 analytes,the quality of Trichosanthes kirilowii from different areas and strains was quite different.Trichosanthis Pericarpium from Hebei province presented significant differences from those in other producing areas.The main different components of which were amino acids and luteoside.CONCLUSION Resource components types in Trichosanthis Pericarpium from different producing areas and strains show little differences,but the contents of these components exhibit significiant differences.The total amounts of resource components and medicinal components in Trichosanthis Pericarpium from traditional medicinal producing areas of Trichosanthes kirilowii with part strains,are higher than those in other producing areas.The results provide references for clinical medication selection and comprehensive utilization of Trichosanthes kirilowii from different areas and strains.
作者
李丹
刘培
张黄琴
李卫文
严辉
尚尔鑫
魏丹丹
董玲
段金廒
LI Dan;LIU Pei;ZHANG Huang-qin;LI Wei-wen;YAN Hui;SHANG Er-xin;WEI Dan-dan;DONG Ling;DUAN Jin-ao(Jiangsu Collaborative Innovation Center for the Industrialization Process of Traditional Chinese Medicine Resources/National and Local Joint Engineering Research Center for the Industrialization of Traditional Chinese Medicine Resources and Prescription Innovation/Key Laboratory of Jiangsu Province's Prescription High Technology Research, Nanjing University of Chinese Medicine, Nanjing, 210023, China;Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei, 230031, China)
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2020年第5期607-614,共8页
Journal of Nanjing University of Traditional Chinese Medicine
基金
中央本级重大增减支项目(2060302)
现代农业技术体系建设专项资金项目(CARS-21)
江苏省“六大人才高峰”高层次人才(JNHB-066)
江苏省高校“青蓝工程”项目。
关键词
瓜蒌皮
栝楼
资源性化学成分
品系差异
产地差异
Trichosanthis Pericarpium
Trichosanthes kirilowii
resources chemical composition
strains differences
production areas differences
