摘要
针对姜黄素在水中溶解性差、稳定性差、难吸收、代谢快等缺点,以姜黄素、金纳米颗粒和哺乳动物红细胞膜为原料,设计开发了红细胞膜仿生纳米载药系统(Au-Cur@RBC)。抗癌药物姜黄素通过疏水作用吸附到利于封装的小尺寸金纳米颗粒表面,然后通过物理挤压的方式将载药纳米粒子包封到红细胞膜囊泡内。结果表明,制备的金纳米粒子尺寸均一度高,分散性良好,平均粒径为13.18 nm。金-姜黄素纳米颗粒中姜黄素的吸附率为86.85%,载药率为20.25%。金纳米颗粒的负载可显著提高姜黄素的稳定性,金-姜黄素纳米颗粒在水溶液中储存30 d后仍保留了52%的姜黄素。同时,暗场显微镜结果表明,红细胞膜涂覆增强了制备的Au-Cur@RBC对暗场显微镜入射光线的折射与衍射能力,从而呈现更清晰的图像。
To overcome the shortcomings of curcumin, such as poor solubility, poor stability, difficult absorption and fast metabolism in water, an erythrocyte membrane bionic nano-drug loading system(Au-Cur@RBC) was designed and developed with curcumin, gold nanoparticles and mammalian erythrocyte membrane as raw materials. The anticancer drug curcumin was adsorbed on the surface of small-sized gold nanoparticles by hydrophobic action, and then encapsulated into erythrocyte membrane vesicles by physical extrusion. The results showed that the prepared gold nanoparticles had good size uniformity and dispersibility, with an average particle size of 13.18 nm. The adsorption rate of curcumin in curcumin-loaded nanoparticles was 86.85%, and the drug loading rate was 20.25%. The loading of gold nano-particles could significantly improve the stability of curcumin, and the gold-curcumin nanoparticles still retained 52% of curcumin after being stored in an aqueous solution for 30 days. At the same time, the results of dark field microscope showed that the erythrocyte membrane improved the ability of prepared Au-Cur@RBC to refract and diffract the incident light of the dark field microscope, thereby presenting a clearer image.
作者
李诗浩
张麟
LI Shihao;ZHANG Lin(College of Chemical Engineering and Technology,Tianjin University,Tianjin 300354,China)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2020年第9期1819-1824,共6页
Fine Chemicals
基金
国家自然科学基金(21978205、91534119)
国家重点研发计划课题(2018YFA0900700)。
关键词
红细胞膜
姜黄素
金纳米粒子
癌症治疗
生物成像
功能材料
erythrocyte membrane
curcumin
gold nanoparticles
cancer treatment
biological imaging
functional materials
