摘要
[目的]观察电针对高脂饮食诱导的胰岛素抵抗肥胖大鼠肠道菌群结构和功能以及体重、进食量、胰岛素敏感性的影响,探讨电针改善胰岛素抵抗肥胖的机制。[方法]48只健康Wistar雄性大鼠,随机挑选8只喂以普通饲料作为正常组,其余40只喂以高脂饲料建立胰岛素抵抗肥胖模型。8周后,造模成功的大鼠随机分为模型组、电针组和假电针组,每组8只。电针组取穴:"丰隆""中脘""关元""足(后)三里"。假电针组给予穴旁5 mm处浅针刺,夹持电极,不通电。每次10 min,隔日一次,共治疗8周。分别在治疗前及治疗2、4、6、8周后测量所有大鼠的体重和24 h进食量;治疗结束后以高胰岛素-正葡萄糖钳夹术测定所有大鼠的胰岛素敏感性[用葡萄糖输注速率(GIR)表示];用ELISA法测定血清胰岛素水平;用宏基因组学检测技术检测肠道菌群的分布和特定菌群、代谢功能。[结果]治疗后,与正常组相比,模型组大鼠的24 h进食量、体重和血清胰岛素均显著升高(P<0.01),而GIR显著降低(P<0.01),模型组大鼠肠道拟杆菌门丰度明显减少、厚壁菌门丰度明显升高;且模型组大鼠的肠道菌群在一般功能预测、碳水化合物的转运与代谢、氨基酸的转运与代谢、能量的产生与转化、脂质的转运与代谢COG蛋白功能聚类均降低;与模型组和假电针组相比,电针组大鼠的24 h进食量、体重和血清胰岛素均显著降低(P<0.01),而GIR显著升高(P<0.01),拟杆菌门丰度有升高趋势,厚壁菌门丰度有降低趋势;电针组以上功能COG蛋白功能聚类均有上升趋势。属水平heatmap显示肠道菌群结构和丰度比较,电针组与正常组肠道菌群有相似性,模型组和假电针组肠道菌群也有相似性,但与电针组与正常组之间差异有统计学意义。COG聚类heatmap比较,电针组和正常组有相似性,模型组和假电针组也有相似性,但与电针组与正常组之间差异有统计学意义。[结论]电针可以有效调控胰岛素抵抗肥胖大鼠肠道菌群的结构和功能,可能是电针改善胰岛素抵抗肥胖的机制之一。
[Objective]To study the effect of electroacupuncture(EA)on improving the structure and function of intestinal microflora in obese rats with insulin resistance,so as to reveal the mechanism of acupuncture on insulin resistance and body weight loss.[Methods]Randomly divide forty-eight male Wistar rats into control group,model group,EA group and sham EA group(n=8 in each group).The model was established by high-fat diet.EA(2 Hz,1 mA)was applied to"Fenglong"(ST40)/"Zusanli"(ST36)and"Zhongwan"(CV12)/"Guanyuan"(CV4),or sham acupoints(about 5 mm beside the acuponts,clamping electrodes but not electrifying)for 10 min,once every other day,3 times per week for a total of 8 weeks.The 24-hour food intake and body weight of all rats were measured.At the 6 th week of intervention,glucose infusion rate(GIR)was detected by hyperinsulin-normal glucose clamp at the end of the intervention.The contents of serum INS was measured by using radioimmunoassay method,while the distribution of intestinal microflora,specific flora and metabolic function were detected by macrogenomics.[Results]After treatment,compared with the control group,the weight and 24-hour food intake and INS in the model group were remarkably increased(P<0.05),while GIR was significantly decreased(P<0.05).In the model group,the abundance of Bacteroides and sclerenchyma decreased significantly,while the general function prediction,carbohydrate transport and metabolism,amino acid transport and metabolism,energy production and transformation,lipid transport and metabolic cog protein functional clustering decreased.Compared with the model group and the sham EA group,the 24-hour food intake,body weight and serum INS in the EA group were significantly reduced(P<0.01),while the GIR was significantly increased(P<0.01),the Bacteroides abundance was increased,while the pachytene abundance was decreased;the functional clustering of the above functional cog protein in the EA group was increased.At the genus level,Heatmap showed the structure and abundance of intestinal flora.The intestinal flora in the EA group was similar to that in the normal group,the intestinal flora in the model group and the sham EA group were also similar,but there was significant difference with the EA group and the normal group.Compared with the normal group,the EA group was similar to the model group and the sham EA group,but there was significant difference with the EA group and the normal group.[Conclusion]EA can effectively reduce appetite and body weight loss in obese rats with insulin resistance,which may be closely related to its effect in improving the structure and function of intestinal microflora.
作者
宋爱群
张阳普
陈瑞
梁凤霞
SONG Ai-qun;ZHANG Yang-pu;CHEN Rui;LIANG Feng-xia(Department of Accupuncture,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430061,China;Hubei Province Academy of Traditional Chinese Medicine,Wuhan 430074,China;Department of Physical Medicine and Rehabilitation,Hubei Provincial Hospital of Integrated Chinese and Western Medicine,Wuhan 430022,China;Department of Integrated TCM and Western Medicine,Union Hospital,Tongji Medical College of Huazhong Science and Technology University,Wuhan 430022,China;Department of Acupuncture and Moxibustion,Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture&Moxibustion,Wuhan 430061,China)
出处
《中国中西医结合消化杂志》
CAS
2020年第9期656-662,共7页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
国家自然科学基金(No:81774420)。
关键词
电针
胰岛素抵抗
肥胖
肠道菌群
electroacupuncture
insulin resistance
obesity
intestinal microflora
