摘要
该文采用邻苯二胺柱前衍生化,建立了超高效液相色谱-串联质谱法测定木瓜发酵饮料中乙二醛、丙酮醛和丁二酮3种α-二羰基化合物含量的方法。样品加水稀释后采用离心超滤进行净化,室温下衍生,衍生产物采用Waters XBridge BEH C 18色谱柱(100 mm×2.1 mm,1.7μm)分离,流动相为乙腈-0.2%(体积分数)乙酸溶液,梯度洗脱,采用电喷雾离子源以正离子多重反应监测模式进行质谱检测。结果表明,3种α-二羰基化合物在0.012~0.900 mg/L线性关系良好,相关系数(r^2)均>0.99,平均加样回收率在86.26%~95.29%,RSD为2.49%~7.31%(n=6)。该方法快速灵敏,适合于木瓜发酵饮料中α-二羰基化合物的检测。
A method for the determination of glyoxal,methylglyoxal and dimethylglyoxal in Fructus chaenomelis fermented beverage by UPLC-MS/MS with o-phenylenediamine pre-column derivatization was established.The sample was diluted with water and purified by centrifugal ultrafiltration method.The derivative products were separated on a Waters XBridge BEH C 18 column(100 mm×2.1 mm,1.7μm)with a gradient elution system,the mobile phase was acetonitrile-0.2%acetic acid solution.Eluted compounds were detected in the electrospray ionization positive mode with multiple reaction monitoring.The results showed that the linear relationship of these threeα-dicarbonyl compounds were good in the range of 0.012-0.900 mg/L,the correlation coefficient(r^2)were greater than 0.99.The average recovery was 86.26%-95.29%with the relative standard deviation(RSD)of2.49%-7.31%(n=6).This method is rapid,sensitive and thus could be successfully applied to the analysis ofα-dicarbonyl compounds in fermented beverage.
作者
陈荣祥
顾雯
罗敏
何婷
尹考
罗春燕
CHEN Rongxiang;GU Wen;LUO Min;HE Ting;YIN Kao;LUO Chunyan(Analytical and Testing Center,Zunyi Medical University,Zunyi 563000,China;School of Pharmaceutical Sciences,Zunyi Medical University,Zunyi 563000,China;School of Laboratory Medicine,Zunyi Medical University,Zunyi 563000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第18期197-202,共6页
Food and Fermentation Industries
基金
国家自然科学基金项目(81760652)
贵州省科技厅基础研究项目(黔科合基础[2016]1172)
波谱与原子分子物理国家重点实验室开放课题(T151601)
遵义医科大学大学生创新创业项目(201751092,ZYDC2018029,2018103)。
关键词
超高效液相色谱
串联质谱法
α-二羰基化合物
乙二醛
丙酮醛
丁二酮
ultra performance liquid chromatography
tandem mass spectrometry
α-dicarbonyl compounds
glyoxal
methylglyoxal
dimethylglyoxal
