摘要
经表皮组织匀浆抽提,无水乙醇分级沉淀,DEAE-Sepharose离子交换层析,Superdex-200凝胶过滤层析,获得电泳纯度的过氧化物酶.该酶比活力、纯化倍数及回收率分别为571975.93 U/mg,51.72,33.80%.该酶总分子量约为37.2 kDa,且为单聚体.该酶最适温度,pH值分别为65℃,6.2,具有耐酸耐热特性.‘忠薯1’过氧化物酶Km值为52.833 mmol/L,且属于乒乓反应.Mg^2+,Cu^2+,Co^2+,Fe^3+对该酶有较强激活作用;Pb2+是甘薯过氧化物酶的有效抑制剂.
Peroxidase(POD)with electrophoretic purity from sweet potato cv.‘Zhongshu1’was obtained by homogenization,ethanol precipitation,DEAE-Sepharose ion exchange chromatograpy and Superdex-200 gel filtration chromatography.Its specific activity,purification fold and activity recovery were 571975.93 U/mg,51.72 and 33.80%,respectively.It had a molecular mass of 37.2 kDa and was a monomer by electrophoresis.Its optimum temperature and pH were 65℃and 6.2,respectively,indicating its good tolerance to acidic and high temperature environments.The Km value of the peroxidase was 52.833 mmol/L,which belonged to ping pong reaction.Mg^2+,Cu^2+,Co^2+and Fe^3+exerted strong positive effects to the enzyme,but Pb^2+could effectively inhibit its activity.
作者
李丰茂
李佳欣
郭小路
傅玉凡
唐云明
LI Feng-mao;LI Jia-xin;GUO Xiao-lu;FU Yu-fan;TANG Yun-ming(School of Life Science,Southwest University/Engineering&Technology Research Center for Swect Potato of Chongqing,Chongqing 400715,China;Fengdu Ecological Environmental Monitoring Station,Fengdu Chongqing 408299,China)
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第10期79-87,共9页
Journal of Southwest University(Natural Science Edition)
基金
现代农业产业技术体系建设专项(CARS-10-C20)
农作物种质资源保护与利用专项(2018NWB036-09-01).
关键词
甘薯
过氧化物酶
分离纯化
性质
sweet potato
peroxidase
purification and isolation
property
