摘要
转录组测序可在各种环境条件下对物种进行高通量测序,通过基因结构分析和功能注释,探讨特定条件下相关基因的功能和表达情况。该研究分别获取低盐胁迫组(盐度0.2)和自然海水组(盐度31)脊尾白虾(Exopalaemon carinicauda)样品,通过Illumina平台进行转录组测序,获得13.92 Gb高质量测序数据,组装得到111618条转录本和72734条Unigenes,其中有22879条Unigenes得到注释,比对到Nr数据库的Unigenes为21931条;筛选出1492条脊尾白虾低盐胁迫差异显著表达基因,包括829条上调基因和663条下调基因,其中有810条差异表达基因得到注释。通过差异表达基因GO功能注释和富集分析及KEGG通路富集分析,锁定大量脊尾白虾在自然海水和淡水环境下的差异表达基因,为深入探讨脊尾白虾在低盐胁迫条件下的生理保护机制提供了技术支撑。
Transcriptome sequencing can be used for high-throughput sequencing of species under various environmental conditions.We explored the function and expression of related genes under specific conditions by gene structure analysis and gene function annotation.E.carinicauda samples at freshwater(salinity 0.2)and natural seawater(salinity 31)were sequenced by Illumina platform,and 13.92 Gb high-quality sequencing data was obtained.111618 transcripts and 72734 unigenes were assembled.A total of 22879 unigenes were annotated,of which 21931 were compared to the Nr database.1492 differentially expressed genes were screened,including 829 up-regulated genes and 663 down-regulated genes,810 of which were annotated.According to the GO function annotation,enrichment analysis and enrichment analysis of KEGG pathway of differentially expressed gene,we excavated differentially expressed genes of the natural seawater and freshwater environments,which provides technical support for further research on the physiological protection mechanism of E.carinicauda under low salinity stress.
作者
沈晔
王兴强
曹梅
郑年昊
陈百尧
秦传新
SHEN Ye;WANG Xingqiang;CAO Mei;ZHENG Nianhao;CHEN Baiyao;QIN Chuanxin(College of Marine Life and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;Lianyungang Agricultural and Rural Bureau,Lianyungang 222005,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2020年第5期19-32,共14页
South China Fisheries Science
基金
江苏省研究生科研与实践创新计划项目(SJCX18_0927)
江苏省海洋生物技术重点实验室开放基金(HS16005)
广东省渔业生态环境重点实验室开放基金(FEEL_2019_3)
连云港市“海燕计划”。
关键词
脊尾白虾
低盐胁迫
转录组分析
差异表达基因
Exopalaemon carinicauda
Low salinity stress
Transcriptome analysis
Differentially expressed gene
