摘要
以簕菜茶为原料,考察了超声辅助技术提取其绿原酸工艺。结合单因素和正交试验,优化得到最佳提取工艺条件:乙醇浓度50%,料液比1:60(g/mL),提取时间80 min,超声温度55℃。在此条件下,绿原酸平均提取率3.77%,相对标准偏差(RSD)0.84%(n=3),加标平均回收率99.22%,RSD 0.60%(n=6)。该试验方法操作简便、准确、稳定,且成本较低,可为进一步研究簕菜茶中绿原酸提供理论依据。
Using Acanthopanax trifoliatus tea as raw material,ultrasonic-assisted technology for extraction of chlorogenic acid was investigated.Combining single factor and orthogonal experiments,the optimum extraction condition was 50% of ethanol concentration,1:60(g/mL)of solid-liquid ratio,80 min of extraction time,and 55℃ of ultrasonic temperature.Under the optimum condition,the average extraction rate of chlorogenic acid was 3.77% and the relative standard deviation(RSD)was 0.84%(n=3).Besides,the average recovery rate was 99.22%,and the RSD was 0.60%(n=6).The test method is simple,accurate and stable,and lower cost,which could provide a theoretical basis for further research on chlorogenic acid in Acanthopanax trifoliatus tea.
作者
郭蒙
黄绍敏
石慧丽
贺贵才
高林晓
杨再波
GUO Meng;HUANG Shao-min;SHI Hui-li;HE Gui-cai;GAO Lin-xiao;YANG Zai-bo(School of Chemistry and Chemical Engineering,Qiannan Normal College for Nationalities,Duyun 558000,China;National Medicinal Plant Resources Development Engineering Research Center of General University of Guizhou Province,Duyun 558000,China;China National Research Institute of Food&Fermentation Industries Corporation Limited,Beijing 100015,China)
出处
《茶叶通讯》
北大核心
2020年第3期472-477,共6页
Journal of Tea Communication
基金
贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]440)
黔南民族师范学院校级科研项目(qnsy2017016)
贵州省普通高校民族药用植物资源开发工程研究中心开放基金(黔教合KY字[2014]227)。
关键词
簕菜茶
绿原酸
超声
提取
正交试验
Acanthopanax trifoliatus tea
Chlorogenic acid
Ultrasound
Extraction
Orthogonal test
