摘要
【目的】了解2017年济宁市某县登革热疫情后,白纹伊蚊幼虫及成蚊对几种常用杀虫剂的抗药性水平,为科学合理使用杀虫剂提供数据支撑。【方法】幼虫测定采用浸液法,成蚊测定采用接触筒法。采集的白纹伊蚊幼虫在实验室饲养繁殖1代后,取三龄末四龄初幼虫以及羽化3~5 d未吸血雌蚊作为测试蚊虫,分别测定幼虫及成蚊的抗药性水平并判断抗药性程度。【结果】2018年济宁市某县主要使用氯菊酯、高效氯氟氰菊酯、cis-氯氰菊酯等拟除虫菊酯类和辛硫磷等有机磷类杀虫剂。白纹伊蚊幼虫对双硫磷、溴氰菊酯、氯菊酯、高效氯氰菊酯4种常见杀虫剂的LC 50值分别为0.006、0.021、0.209、0.016 mg/L,抗药性倍数分别为3.75、52.50、104.50和17.78倍;另外,对双硫磷低抗,对高效氯氰菊酯中抗,对溴氰菊酯和氯菊酯高抗。成蚊对氯菊酯敏感,击倒率为97.62%,死亡率为100%;对残杀威和马拉硫磷可能抗药性,击倒率分别为97.06%和91.89%,死亡率分别是97.06%和93.24%;对溴氰菊酯、cis-氯氰菊酯、高效氯氟氰菊酯、高效氯氰菊酯为抗药性,击倒率分别是88.00%、95.31%、91.04%、97.10%,死亡率均小于80.00%。【结论】济宁市某县白纹伊蚊幼虫、成蚊对常用杀虫剂已产生不同程度的抗药性,应定期监测白纹伊蚊对杀虫剂的抗药性,科学合理地使用杀虫剂,延缓抗药性产生。
[Objective]To investigate the resistances of larva and adult of Aedes albopictus to common insecticides in a county of Jining City,after the dengue fever outbreak in 2017,and to provide scientific support for rational use of insecticides.[Methods]The insecticide resistance was tested with exposure tubes for adult mosquitoes and dipping method for larvae.After collecting the larvae and breeding for one generation,the resistances of larvae were determined with the late third-instar or early fourth-instar larvae,and the resistance of adults was tested with the female mosquitoes at 3-5 days post eclosion.[Results]The insecticides mainly used in the county of Jining City in 2018 were pyrethroids such as permethrin,β-cyhalothrin,α-cypermethrin,and organic phosphorus pesticides such as phoxim.The median lethal concentration(LC50)values in the larva to temephos,deltamethrin,permethrin,beta-cypermethrin were 0.006 mg/L(RR=3.75),0.021 mg/L(RR=52.50)、0.209 mg/L(RR=104.50)、0.016 mg/L(RR=17.78),with the lowest resistance to temephos and the highest to permethrin.Adult mosquitoes were sensitive to permethrin,with a knockdown rate of 97.62%and a mortality rate of 100%.When exposed to propoxur and malathion,the knockdown rates were 97.06%and 91.89%,the mortality rates were 97.06%and 93.24%.Aedes albopictus larvae were resistant to deltamethrin,α-cypermethrin,β-cyhalothrin,β-cypermethrin,with the knockdown rates of 88.00%,95.31%,91.04%,97.10%,and the mortality rates were all less than 80.00%.[Conclusion]The larvae and adult mosquitoes of Aedes albopictus in the county of Jining City have developed different degrees of resistance to commonly used insecticides.The resistance should be under regular monitoring and the use of insecticides should be scientific and rational to delay the resistance production.
作者
杨琳琳
白靓
陆瑶
刘鲁宏
YANG Lin-lin;BAI Jing;LU Yao;LIU Lu-hong(Jining Center for Disease Control and Prevention,Jining,Shandong 272000,China)
出处
《上海预防医学》
CAS
2020年第8期651-654,共4页
Shanghai Journal of Preventive Medicine
关键词
登革热
白纹伊蚊
抗药性
Dengue
Aedes albopictus
insecticide resistance
