摘要
[目的]了解重庆市家禽屠宰环节金黄色葡萄球菌的污染情况,为防控金黄色葡萄球菌食源性疾病的发生提供参考依据。[方法]按照GB 4789.10—2016《食品安全国家标准食品微生物学检验金黄色葡萄球菌检验》对重庆市屠宰环节400份样品进行检测,同时随机选择40株金黄色葡萄球菌进行药敏试验和致病性分析。[结果]屠宰环节金黄色葡萄球菌污染程度达59.25%,秋季较夏季污染程度重,为67.00%,胴体比环境污染重,为65.71%,烫洗煺毛和净膛环节污染风险较重均达60%以上。40株分离株中毒素基因检出率为65.00%,总耐药率为95.00%,主要对青霉素、四环素、红霉素和克林霉素耐药;70.00%的菌株同时对3种以上抗生素耐药。[结论]重庆市家禽屠宰环节金黄色葡萄球菌污染较重,部分分离株存在多个毒素基因和多重耐药现象,值得高度重视。
[Objective]To understand the pollution of Staphylococcus aureus in slaughtered poultry products in Chongqing.[Method]According to GB 4789.10-2016 National Food Safety Standard-Food Microbiological Examination S.aureus,a total of 400 samples in Chongqing,40 strains of S.aureus were randomly selected for drug sensitivity test and pathogenicity analysis.[Result]S.aureus pollution reached 59.25%.Autumn was more polluted than summer,67.00%.Carcass was more polluted than environment,65.71%.The pollution risks in ironing and cleaning was more than 60%.In 40 isolates,the detected rate of toxin gene was 65.00%,and the average drug resistance rate was 95.00%.The S.aureus isolates were highly resistant to penicillin,tetracycline,erythromycin and clindamycin,70.00%strains displayed multi-drug resistance.[Conclusion]The pollution of S.aureus in slaughtered poultry products in Chongqing is serious,some isolates had multiple toxin genes and multi drug resistance,which should be paid more attention to.
作者
谢建华
叶洁莹
王棋
梁望旺
杨泽林
熊仲良
曾政
XIE Jian-hua;YE Jie-ying;WANG Qi(Chongqing Animal Disease Prevention and Control Center,Chongqing 401120)
出处
《安徽农业科学》
CAS
2020年第20期163-165,共3页
Journal of Anhui Agricultural Sciences
基金
社会民生类重点研发项目(cstc2018jscx-mszdX0049)
重庆市技术创新与应用发展专项(cstc2019jscx-msxmX0370)。
关键词
金黄色葡萄球菌
禽产品
细菌污染
Staphylococcus aureus
Poultry products
Bacterial contamination
