摘要
目的:探讨RA相关区域家族1A(RASSF1A)基因甲基化及血浆骨髓细胞瘤病病毒致癌基因同源物(MYC)抗体在宫颈癌诊断中的价值。方法:选取2017年1月~2018年6月本院收治的64例宫颈癌患者为研究对象,同期健康体检女性45例为对照组。采用巢式甲基化特异性PCR(nMSP)法检测两组妇女RASSF1A基因甲基化状态,采用酶联免疫吸附实验法检测血浆MYC抗体水平,分析二者检测诊断宫颈癌价值。结果:64例宫颈癌患者血浆检测出RASSF1A基因启动子甲基化31例(48.4%),45例对照组女性均未检测出RASSF1A基因启动子甲基化,两组存在差异(P<0.05);宫颈癌组患者血浆MYC抗体水平高于对照组(P<0.05);宫颈癌患者RASSF1A基因甲基化与患者临床分期及淋巴结转移有关,血浆MYC抗体水平与临床分期及肿瘤直径有关(均P<0.05);血浆MYC抗体水平诊断宫颈癌的曲线下面积为0.611,敏感度为35.9%,特异度为84.4%。结论:检测RASSF1A基因甲基化及血浆MYC抗体在宫颈癌诊断中有一定临床价值。
Objective: To investigate the value of the methylation of RA-related regional families 1 A(RASSF1 A) gene combined with plasma MYC antibody for diagnosing cervical cancer. Methods: 64 women with cervical cancer were selected in study group from January 2017 to June 2018, and 45 healthy women were selected in control group during the same period. Nested methylation specific PCR(nMSP) was used to detect the methylation status of RASSF1 A gene of women in the two groups, and the level of plasma MYC antibody was detected by enzyme-linked immunosorbent assay(ELISA). The value of detection and diagnosis the methylation status of RASSF1 A gene and plasma MYC antibody level for cervical cancer was analyzed. Results: There were 31(48.4%) women with promoter methylation of RASSF1 A gene in the study group, while there were no woman with promoter methylation of RASSF1 A gene, which had significant difference between the two groups(P<0.05). The plasma MYC antibody level of women in the study group was significant higher than that of women in control group(P<0.05). Methylation of RASSF1 A gene of women with cervical cancer was related to their clinical stage and lymph node metastasis, and plasma MYC antibody level of women with cervical cancer was related to clinical stage and tumor diameter(all P<0.05). The area under the curve, the sensitivity, and the specificity of plasma MYC antibody level for diagnosing cervical cancer were 0.611, 35.9%, and 84.4%, respectively. Conclusion: Methylation of RASSF1 A gene combined with plasma MYC antibody level for diagnosing cervical cancer has some clinical value.
作者
程晓春
周欣
李晖
周涛琪
庞林荣
陈俊
CHENG Xiaochun;ZHOU Xin;LI Hui;ZHOU Taoqi;PANG Linrong;CHEN Jun(Yinzhou People's Hospital,Ningbo,Zhejiang Province,315040)
出处
《中国计划生育学杂志》
2020年第10期1646-1649,共4页
Chinese Journal of Family Planning
