摘要
目的观察四神丸对腹泻型肠易激综合征(IBS-D)模型大鼠脊髓中胃促生长素(Ghrelin)及其受体GHSR表达的影响。方法将SPF级SD大鼠先随机分为正常组和造模组,造模组采用番泻叶灌胃加避水应激法制备脾肾阳虚型IBS-D大鼠模型,待模型复制成功后再将造模组随机分为模型组、得舒特组和四神丸高、中、低剂量组;灌胃14 d后,免疫组化法检测脊髓组织Ghrelin、GHSR的阳性表达,RT-PCR法检测脊髓中Ghre⁃lin、GHSR的mRNA表达。结果模型组脊髓中Ghrelin、GHSR阳性细胞数增多、mRNA表达升高,与正常组比较,差异均有统计学意义(P<0.01);四神丸高、中剂量组脊髓Ghrelin、GHSR的阳性细胞数减少、mRNA表达降低,与模型组比较,差异有统计学意义(P<0.05或P<0.01);且四神丸高剂量组对脊髓Ghrelin、GHSR阳性细胞数表达的影响优于其低剂量组(P<0.05)。结论四神丸对IBS-D模型大鼠的作用可能与降低脊髓中Ghrelin、GHSR的表达有关。
Objective:To observe the effect of Sishen Pills on the expression of Ghrelin and its receptor GHSR in the spinal cord of rats with diarrhea irritable bowel syndrome(IBS-D)model.Methods:SPF SD rats were randomly divided into the normal group and model group.The IBS-D model rats of asdthenic splenonephro-yang were established by intragastric administration of senna leaf and water avoidance stress method.Then the successfully modeled rats were randomly divided into the model group,Dicetel group and Sishen Pills high,medium and low dose groups.After intragastric administration for 14 days,the positive expression of Ghrelin,GHSR in spinal cord tissue were detected by immunohistochemistry,and the expression of Ghrelin,GHSR mRNA in spinal cord were detected by RT-PCR method.Results:The number of Ghrelin,GHSR positive cells and the expression of mRNA in spinal cord were increased in the model group,and the difference was statistically significant compared with normal group(P<0.01).The number of Ghrelin,GHSR positive cells and mRNA expression in spinal cord of the high and middle dose group of Sishen pill were decreased,which was statistically significant compared with the model group(P<0.05 or P<0.01).The effect of high dose of Sishen Pills on the number of Ghrelin,GHSR positive cells in spinal cord were better than that in low dose(P<0.05).Conclusion:The effect of Sishen Pills on the IBS-D rats may be related to the reduction of the expression of Ghrelin,GHSR in the spinal cord.
作者
蔺晓源
邓娜
夏旭婷
蔡莹
刘富林
Lin Xiaoyuan;Deng Na;Xia Xuting;Cai Ying;Liu Fulin(The First Hospital of Hunan University of Chinese Medicine,Hunan,Changsha 410007,China)
出处
《中国中医急症》
2020年第10期1735-1738,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
国家自然科学基金项目(81403380)
湖南省自然科学基金项目(2020JJ5432)
湖南省卫生健康委科研课题(20200541)
湖南中医药大学中医学国内一流建设学科开放基金(2018ZYX21)。
