摘要
为实现萱藻丝状体的快速扩增,本实验以萱藻丝状体为研究对象,应用实验生态学方法对三种充气条件(充空气、连续充CO2以及基于培养液pH稳定在7.5~8.0条件的间歇充CO2)下气升式光生物反应器中萱藻丝状体的生长、生理及发育进行了比较研究。结果显示,间歇充CO2可显著提高萱藻丝状体的日均增长率,扩增18 d日均增长率为(24.15±2.34)%,显著高于充空气与连续充CO2组;间歇充CO2条件下萱藻丝状体代谢旺盛,生长良好,光合放氧及呼吸耗氧速率分别为(25.99±1.88)和(15.98±0.97)μmol O2·g^-1 FW·h^-1,较连续充CO2组分别提高了38.23%和55.05%,较充空气组分别提高了57.91%和43.46%;间歇充CO2可促进萱藻丝状体孢子囊的发育,诱导18 d孢子囊比例可达(51.03±0.78)%,显著高于充空气组与连续充CO2组;间歇充CO2条件下扩增获得的丝状体游孢子放散量及附着率最高,经阴干刺激,放散2 h后孢子放散量为(11.33±1.33)×104 ind/mg FW,48 h后附着率为(90.67±2.40)%,较连续充CO2组分别提高了17.24%和3.82%,较充空气组分别提高了99.82%和5.54%。
In this experiment,the growth,physiology and development of filaments of Scytosiphon lomentaria in airlift photobioreactor under three kinds of aeration condition were studied,in which air-aerated,continuously aerated with CO2 and intermittently aerated with CO2 that could stabilize the pH in 7.5~8.0 were implement.Results indicated that intermittently aerated with CO2 could significantly increase the average daily growth rate of filaments of S.lomentaria.After 18-day culture,the average daily growth rate of filaments in intermittently aerated with CO2 group could reach(24.15±2.34)%which was significantly higher than those in air-aerated and continuously aerated with CO2 group.Moreover,under intermittently aerated with CO2 condition,the metabolism of filaments of S.lomentaria were vigorous.The rate of photosynthetic oxygen evolution and respiration oxygen consumption of filaments of S.lomentaria in this condition could come up to(25.99±1.88)and(15.98±0.97)μmol O2·g^-1 FW·h^-1,respectively,which were enhanced by 38.23%and 55.05%comparing to the continuously aerated with CO2 group as well as 57.91%and 43.46%comparing to the air-aerated group.In addition,intermittently aerated with CO2 could promote the development of unilocular sporangia.After 18-day induction,ratios of unilocular sporangia under intermittently aerated with CO2 condition could reach(51.03±0.78)%,which were significantly higher than those in air-aerated group and continuously aerated with CO2 group.Furthermore,the filaments amplified in the intermittently aerated with CO2 condition had the highest amount of released spores and spore attachment rate.The amount of released spores in this group was(11.33±1.33)×104 ind/mg FW after 2 h of the spores releasing,which was enhanced by 17.24%and 99.82%comparing to the continuously aerated with CO2 group and air-aerated group,respectively.What is more,after 48h of spore attachment,the attachment rate in intermittently aerated with CO2 group was(90.67±2.40)%,which was enhanced by 3.82%and 5.54%comparing to the continuously aerated with CO2 group and air-aerated group,respectively.
作者
庄英瑞
宫相忠
杨儒谦
高伟
张敏
毕萌
ZHUANG Ying-Rui;GONG Xiang-Zhong;YANG Ru-Qian;GAO Wei;ZHANG Min;BI Meng(College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China)
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第12期70-77,共8页
Periodical of Ocean University of China
基金
山东省重点研究发展计划项目(2016GSF115042)资助。
关键词
萱藻
丝状体
气升式光生物反应器
CO2
生长
光合作用
呼吸作用
发育
Scytosiphon lomentaria
filaments
airlift photobioreactor
CO2
growth
photosynthesis
respiration
development
