摘要
目的建立快速测定肉苁蓉饮片中沙苁蓉掺伪比例的分析方法,为有效地监管、控制肉苁蓉饮片质量提供保障。方法首先采用四分法从混合均匀的待测饮片中取样,获得混合样品。基于沙苁蓉饮片的性状特征,从样品中挑选出部分沙苁蓉样品。进一步建立沙苁蓉中金石蚕苷的高效液相色谱分析方法,考察金石蚕苷峰面积与掺伪比例二者之间的关系,建立沙苁蓉掺伪比例计算公式。测定混合样品和沙苁蓉样品中金石蚕苷的峰面积,通过本实验建立的沙苁蓉掺伪比例计算公式,即可计算出肉苁蓉饮片样品中沙苁蓉的掺伪比例。结果3批肉苁蓉饮片中沙苁蓉掺伪比例为18.6%,2.8%和3.6%。结论该方法分析速度快,测定结果准确,灵敏度高,可为肉苁蓉饮片中沙苁蓉掺伪情况评价提供依据,也可为其他中药饮片的掺伪情况分析提供参考。
OBJECTIVE To develop an analytical method for rapid determination of adulterated proportion of Cistanche sinensis in the Cistanches Herba pieces.METHODS First,mixed sample was obtained by sample quartering from Cistanches Herba pieces.Based on the traits of the Cistanche sinensis pieces,some Cistanche sinensis pieces were partly picked out.Then,a high-performance liquid chromatography method for determination of poliumoside in the Cistanche sinensis was developed,and calculation formula of adulterated proportion was established by investigating relationship between the area of poliumoside and adulterated proportion.Finally,adulterated proportion could be calculated by putting the areas of poliumoside in mixed sample and some Cistanche sinensis pieces into the established formula.RESULTS Adulterated proportion of 3 samples were 18.3%,3.1%and 5.0%.CONCLUSION The method is efficient,accurate and sensitive,which can provide a basis for adulteration detection of Cistanches Herba pieces and other Chinese herbal pieces.
作者
高妍
过立农
马双成
刘杰
郑健
昝珂
GAO Yan;GUO Li-nong;MA Shuang-cheng;LIU Jie;ZHENG Jian;ZAN Ke(National Institutes for Food and Drug Control,Beijing 100050,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2020年第19期1629-1633,共5页
Chinese Pharmaceutical Journal
基金
国家科技重大专项资助(2018ZX09735-006)
中医药行业科研专项“中药饮片质量保障系统研究(一)”资助(201507002)。
关键词
肉苁蓉
沙苁蓉
掺伪比例
高效液相色谱法
Cistanches Herba
Cistanche sinensis
adulterated proportion
HPLC
