摘要
目的探讨黄芪总黄酮(total flavonoids of astragalus,TFA)改善四氯化碳(CCl4)诱导的大鼠肝纤维化效果及抗炎抗氧化作用机制。方法大鼠分为正常组、模型组、TFA低剂量组和TFA高剂量组。除正常组外,其余各组给予CCl4橄榄油皮下注射,每周2次,共8周。造模4周后,模型组给予生理盐水灌胃,TFA低剂量组和TFA高剂量组给15mg/kg和30mg/kg的TFA灌胃,1次/d,治疗4周后分别取血清及肝组织标本。血清检测ALT、AST、ALB水平;肝组织做病理学检查;qPCR法检测相关炎症因子和法尼酯衍生物X受体(farnesoid X receptor,FXR)的mRNA表达水平;Western blot检测NF-κB、FXR等蛋白表达变化;试剂盒检测丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)水平。结果与模型组相比,TFA低剂量组和TFA高剂量组中肝脏脂肪变性明显减轻、炎症细胞浸润减少及肝纤维化减轻;肝功能相关指标AST、ALT、ALB明显好转;相关炎症因子的mRNA和蛋白下降;FXR的表达明显上调;肝组织中MDA水平明显降低,GSH、SOD表达水平增高。结论TFA具有抗炎、抗氧化作用,能够有效地延缓四氯化碳诱导的肝纤维化进程,其机制可能与调控FXR的表达相关。
Objective To investigate the effect of total flavonoids of astragalus(TFA)on CCL4 induced hepatic fibrosis in rats and the mechanism of anti-inflammatory and anti-oxidation.Methods The rats were randomly divided into four groups:control group,model group,low dose TFA group and high dose TFA group.All rats except those in control goup were given subcutaneous injection of CCL4 and olive mixture,twice a week for 8 weeks.After 4 weeks,the rats in model group were treated with normal saline,low dose and high dose TFA group were administered with 15mg/kg and 30mg/kg TFA by gavage each day.After 4 weeks of treatment,serum and liver tissue samples were collected to detect the levels of AST,ALT and ALB.HE staining was used to evaluate the hepatic fibrosis.The mRNA expression levels of inflammatory cytokines and farnesoid X receptor(FXR)were analysed by Real Time PCR.The protein expression levels of NF-κB and FXR were detected by Western blot.The levels of MDA,GSH and SOD in the liver were quantified with assay kits.Results The results showed that low dose and high dose TFA treatment could significantly alleviate the fatty degeneration of liver,reduce the infiltration of inflammatory cells and suppress hepatic fibrosis compared with model group.The levels of AST,ALT and ALB were improved significantly in low dose and high dose TFA group.Compared with model group,the mRNA and protein expression levels of inflammatory cytokines were down-regulated and the levels of FXR were up-regulated,moreover,the level of MDA was decreased,and GSH and SOD were increased in TFA treatment group.Conclusion TFA has anti-inflammatory and anti-oxidation effects,which can effectively delay the process of CCL4 induced hepatic fibrosis,and its mechanism may be related to the regulation of FXR expression.
作者
谌卫龙
李卫明
张守华
雷俊
涂小飞
曾俊权
CHEN Weilong;LI Weiming;ZHANG Shouhua;无(Department of General Surgery;Department of Clinical Laboratory,Jiangxi Provincial Children's Hospital,Nanchang,Jiangxi Province,Jiangxi,330006,China;Medicine Department of Jinggangshan University,Ji’an,343009,China)
出处
《江西医药》
CAS
2020年第10期1404-1407,1416,共5页
Jiangxi Medical Journal
基金
江西省卫生健康委员会科技计划项目,编号20195590
江西省高校人文社会科学研究项目,编号GL1569。
关键词
黄芪总黄酮
肝纤维化
法尼酯衍生物X受体
抗氧化
Total flavonoids of astragalus
Hepatic fibrosis
Farnesoid X receptor
Antioxidant
