摘要
以河蚬肉为蛋白源,添加中性蛋白酶进行酶解,采用葡聚糖凝胶G-50分离纯化得抗氧化肽,根据螯合最佳工艺制备抗氧化肽—锌螯合物,采用茚三酮法和硫化钠法对螯合产物进行定性分析,并通过紫外、红外、荧光光谱、X射线衍射和扫描电镜对产物结构进行分析。结果表明:所得螯合产物是一种抗氧化肽—锌螯合物;抗氧化肽中的羧基、氨基及酰胺键均参与了螯合反应,反应前后肽结构发生了显著变化;根据已得图谱分析可初步预测河蚬抗氧化肽—锌螯合物的结构。
Using clam meat as protein source,the hydrolysate was obtained by enzymehydrolysis with neutral protease.Antioxidant peptides were then separated and purified from the enzymatich-ydrolysate of Corbicula fluminea by using glucose gel G-50.Zinc-binding antioxidant peptides were prepared according to optimal process.Qualitative analysis of chelate products was carried out byninhydrin method and sodium sulfide method.Ultraviolet spectra,Fourier infrared and fluorescent light spectra,X-radiation diffraction and scanning electron microscopy were applied to predict the structure of the chelate products.The results showed that the product powder is a kind of polypeptide zinc chelate.The carboxyl,amino and amide bonds of the polypeptide all participate in the chelation reaction,and the structure of the peptide changed significantly after the reaction.The structure of the antioxidant peptide-zinc chelate from Corbicula fluminea can was deduced preliminarily by analysis data.
作者
刘晶晶
徐蕴桃
朱晨慧
黄倩倩
韩曜平
王雪锋
张然
戴阳军
LIU Jing-jing;XU Yun-tao;ZHU Chen-hui;HUANG Qian-qian;HAN Yao-ping;WANG Xue-feng;ZHANG Ran;DAI Yang-jun(Department of Biology and Food Engineering,Changshu Institute of Technology,Changshu,Jiangsu 215500,China)
出处
《食品与机械》
北大核心
2020年第10期28-31,48,共5页
Food and Machinery
基金
江苏省苏州市应用基础研究计划项目(编号:SNG2017043)。
关键词
河蚬
抗氧化肽
锌
制备
结构表征
Corbicula fluminea
antioxidant peptide
Zinc
preparation
structure characterization
