摘要
肉及其副产物中含有多种蛋白质,这些蛋白质经过酶解、微生物发酵或胃肠道消化后,产生多种有益于人体的活性肽,如降血压肽(血管紧张素转化酶抑制肽)、抗氧化肽、抗菌肽和二肽基肽酶抑制肽等。肽的生物活性逐渐被人们重视,但其提取和筛选仍然受到分离鉴定技术的限制。计算机分析技术能够很好地评估及分离鉴定蛋白质作为活性肽前体蛋白的能力,模拟酶及化学作用对蛋白质的水解,预测肽序列的活性及其化学性质。本文对肉源性活性肽进行综述,并介绍计算机分析技术在分离鉴定活性肽中的应用。
Meat and meat processing by-products contain a variety of proteins,which can be used to produce various bioactive peptides that are beneficial to the human body after enzymatic hydrolysis,microbial fermentation or gastrointestinal digestion,such as antihypertensive peptide(angiotensin converting enzyme inhibitory peptide),antioxidant peptide,antibacterial peptide,and dipeptidyl peptidaseⅣinhibitory peptide.Although increasing attention has been paid to the bioactivities of peptides,extraction and screening of peptides are still limited by the existing purification and identification techniques.In silico analysis can characterize the potential of proteins as precursors of bioactive peptides,simulate proteolysis catalyzed by enzymatic or chemical action,and predict the activity of peptide sequences and their chemical properties.In this paper,bioactive peptides derived from meat proteins are reviewed,as well as the application of in silico analytical techniques in the separation and identification of bioactive peptides.
作者
汤兴宇
扈麟
吴中琴
周明玺
钟嫒嫒
王钰雯
廖奕扬
田星
王远亮
TANG Xingyu;HU Lin;WU Zhongqin;ZHOU Mingxi;ZHONG Aiai;WANG Yuwen;LIAO Yiyang;TIAN Xing;WANG Yuanliang(College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China;College of Pharmacy,Hunan University of Chinese Medicine,Changsha 401208,China)
出处
《肉类研究》
北大核心
2020年第9期88-95,共8页
Meat Research
基金
2019年湖南省自然科学基金青年科学基金项目(2019JJ50450)
2019年湖南省农业产业技术体系项目(湘农发2019-105)
主要农产品质量安全检测与关键控制技术研究与示范项目(2018XK2006)
基于大数据的生猪养殖与优质猪肉加工关键技术研究与应用示范项目(2017XK2058)
湖南农业大学“双一流”学科建设项目(SYL2019061)。
关键词
肉源性活性肽
蛋白质
酶解
计算机分析技术
bioactive peptide derived from meat
protein
enzymolysis
in silico analysis
