摘要
目的建立柳树花粉引起的过敏性鼻炎小鼠模型,并对其进行特异性脱敏治疗。方法提取柳树花粉蛋白,对小鼠腹腔注射并滴鼻激发,建立过敏性鼻炎小鼠模型;通过递增变应原剂量对小鼠皮下注射,观察小鼠行为学症状、鼻黏膜组织形态学变化和检测血清sIgE的含量,探索特异性脱敏疗法的效果。结果治疗前小鼠出现搔鼻、打喷嚏、流鼻涕等过敏症状,鼻黏膜中嗜酸性粒细胞明显多于对照组,血清sIgE含量高于激发前,成功建立了过敏性鼻炎小鼠模型;治疗后小鼠过敏症状逐渐减轻至消失,鼻黏膜中嗜酸性粒细胞逐渐减少,血清sIgE含量减少。结论柳树花粉能够建立过敏性鼻炎小鼠模型,且特异性脱敏法可将其治疗。
Objective Establish mouse models of willow pollen allergic rhinitis and immune tolerance treatment of the mouse models.Method Pollen protein was extracted and mouse models of allergic rhinitis was established by intraperitoneal injection of pollen protein and nasal stimulation.By subcutaneous injection of increasing dosage of allergen into mice,the behavioral symptoms of mice were observed,the morphological changes of nasal mucosa were compared,and the content of specific sIgE in serum was detected,so as to study the therapeutic effect of specific desensitization therapy on allergic rhinitis.Result Before treatment,allergic symptoms such as scratching,sneezing and runny nose appeared in mice.Eosinophilsin nasal mucosa were significantly more than those in control group,and the content of specific sIgE in serum was higher than that before stimulation.These results indicate that the allergic rhinitis mouse models induced by willow pollen has been successfully established.After treatment,allergic symptoms of mice gradually decreased to disappear,eosinophils in nasal mucosa gradually decreased,and specific sIgE content in serum decreased.Conclusion Willow pollen can establish allergic rhinitismodelsin mice,and specific desensitization method could treat allergic rhinitis in mice.
作者
罗兆莉
轩冉
莘琳琳
孙其玉
董晏榕
任其奎
杨帆
田兆菊
LUO Zhaoli;XUAN Ran;SHEN Linlin;SUN Qiyu;DONG Yanrong;REN Qikui;YANG Fan;TIAN Zhaoju(Department of Public Health,Shandong First Medical University,Shandong Academy of Medical Sciences,Tai’an 271016,China;Department of Pathology,Second Affiliated Hospital of Shandong First Medical University,Shandong Academy of Medical Sciences,Tai’an 271016,China)
出处
《实验动物科学》
2020年第4期10-14,共5页
Laboratory Animal Science
基金
2017年国家级大学生创新创业训练计划立项项目(No.201710439211)。
关键词
过敏性鼻炎
特异性脱敏法
柳树花粉
免疫治疗
Allergic rhinitis
Specific desensitization method
Willow trees pollen
immunotherapy
