摘要
目的观察外源性补充重组人胰岛素样生长因子-1(human recombinant insulin-like growth factor-1,rhIGF-1)对氧诱导早产儿视网膜病变(retinopathy of prematurity,ROP)的防治效果。方法把生后7 d的新生昆明小鼠随机分成3组:高氧rhIGF-1组、空气对照组和高氧盐水组,每组16只。空气对照组在正常环境中饲养,高氧rhIGF-1组与高氧盐水组于出生第7天(P7)放入55%~65%氧浓度的氧箱中饲养,12 d后出箱,高氧rhIGF-1组分别于P7、P8下午同一个时间点分别注射rhIGF-1一次,每次0.5 mg/kg;而高氧盐水组与空气对照组分别注射相同剂量生理盐水,于P19、P24各处死8只小鼠摘取眼球,ADP酶染色、视网膜铺片进行观察视网膜上血管的发育情况,并行HE染色,观察突破入视网膜内界膜的血管内皮细胞核计数。每天测量各组小鼠体重增长情况。统计学方法采用t检验、ANOVA分析、LSD多重比较。结果比较各组体重增长的情况,空气对照组的体重高于高氧rhIGF-1组和高氧盐水组,差异均有统计学意义(P<0.05,t值分别为3.572,7.553),提示高氧对小鼠体重增长有抑制作用;P19高氧rhIGF-1组小鼠体重增长速度高于高氧盐水组,差异均有统计学意义(P<0.05,t=3.980)。显示rhIGF-1干预下,能促进高氧环境中小鼠的体重增长。将高氧rhIGF-1组与高氧盐水组比较:突破视网膜内界膜的血管内皮细胞核数明显减少,差异有统计学意义(P<0.05);视网膜血管在P19走行较自然,周边血管闭塞不明显,周边血管覆盖率高,P24未见明显新生血管生成。结论本研究结果显示外源性补充rhIGF-1能有效促进小鼠体重的增加,可能减轻高氧对视网膜的损伤,有望成为ROP防治的新手段。
Objective To observe the effect of exogenous supplementation of human recombinant insulin-like growth factor-1(rhIGF-1)in oxygen-induced retinopathy neonatal mice model.Methods Kunming mice at 7 days after birth were randomly divided into 3 groups:hyperoxia rhIGF-1 group,air control group,and hyperoxia saline group,with 16 mice in each group.Mice in the air control group were assessed to room air.Mice at 7 days after birth in the hyperoxia saline groups and hyperoxia rhIGF-1 group were assessed to expose to 55%-65%oxygen for 12 days.At postnatal day 7 and 8,mice in the hyperoxia rhIGF-1 group received 0.5 mg/kg of rhIGF-1.While air control group and hyperoxia saline group received the same dose of normal saline.At day 19 and 24,8 mice were put to death,whose eyeballs were extirpated and ADP enzyme staining and stretched preparation of retina for observing retinal blood vessels division.HE dyeing was used for counting retinal vascular endothelial cells.Weight growth in each group of mice was monitored every day.Statistical methods were carried out by t-test,ANOVA analysis and LSD multiple comparison.Results Body weight gain was compared in each group.Body weight of the control group growed faster than hyperoxia rhIGF-1 group and hyperoxia saline group with statistical significance(P<0.05,t=3.572,7.553).It showed that hyperoxia has an inhibitory effect on weight gain in mice.P19 mice in the hyperoxia rhIGF-1 group gained weight faster than the hyperoxia saline group with statistical significance(P<0.05,t=3.980).It showed that rhIGF-1 could promote the weight gain of mice in high-oxygen environment.The hyperoxia rhIGF-1 group was less than hyperoxia saline group in vascular endothelial cells extending into the inner boundary membrane of retina with statistical significance(P<0.05).Retinal vessel was normal at P19 and peripheral vascular occlusion was not obvious and peripheral vascular coverage was high.At P24,neovascularization on retina was not obvious.Conclusion Exogenous complementing of rhIGF-1 early can effectively promote weight growth of mice,and may mitigate the damage for the retina caused by hyperoxia,and is expected to be a prophylaxis and treatment method for ROP.
作者
李晓香
李丽华
李秋平
Li Xiaoxiang;Li Lihua;Li Qiuping(Beijing Luhe Hospital,Capital Medical Univercity,Beijing 101100,China;Neonatal Intensive Care Unit of Bayi Children's Hospital,Seventh Medical Center of PLA General Hospital,Beijing 100700,China)
出处
《发育医学电子杂志》
2020年第4期313-317,共5页
Journal of Developmental Medicine (Electronic Version)
基金
国家自然科学基金(81671504)。
