摘要
目的探讨血清长链非编码RNA生长停滞特异基因5(LncRNA GAS5)水平与老年冠状动脉粥样硬化性心脏病(冠心病)在接受经皮冠状动脉介入治疗(PCI)后出现支架内再狭窄的关系,并采用受试者工作曲线(ROC)评估血清LncRNA GAS5水平对老年冠心病PCI后支架内再狭窄的诊断价值。方法选取2016年4月至2018年10月于解放军联勤保障部队第九六〇医院干部病房进行PCI的164例老年冠心病患者为研究对象,依据术后12个月冠状动脉(冠脉)造影复查结果分为两组,支架内、5 mm范围内血管内径狭窄<50%的无再狭窄组(91例),支架内、5 mm范围内血管内径狭窄≥50%的为再狭窄组(73例),再狭窄组根据再狭窄支数分为单支再狭窄组(28例),双支再狭窄组(32例),三支再狭窄组(13例)。研究对象均采用实时荧光定量聚合酶链反应(RT-PCR)检测血清LncRNA GAS5水平。结果与无再狭窄组相比,再狭窄组血清LncRNA GAS5水平较低(P<0.05);单支再狭窄组、双支再狭窄组、三支再狭窄组相比,血清LncRNA GAS5水平依次降低(P<0.05);Spearman相关分析显示,再狭窄组血清LncRNA GAS5水平与老年冠心病PCI后再狭窄支数呈负相关(P<0.05);ROC结果显示,血清LncRNA GAS5诊断老年冠心病患者PCI后支架内再狭窄的曲线下面积为0.866,截断值为1.099,对应的敏感度为86.30%,特异度为73.60%。结论老年冠心病PCI后支架内再狭窄患者血清LncRNA GAS5水平较未发生再狭窄患者低,且与再狭窄支数呈负相关,对老年冠心病PCI后发生支架内再狭窄有一定诊断价值,可能为老年冠心病患者PCI后支架内再狭窄的治疗靶点提供新思路。
Objective To investigate the relationship between level of serum long non-coding RNA growth arrest-specific gene 5(LncRNA GAS5)and in-stent restenosis(ISR)and review the diagnostic value of LncRNA GAS5 level to ISR in elderly patients with coronary heart disease(CHD)after PCI.Methods The patients(n=164)undergone PCI were chosen from Cadre Ward in the 960th Hospital of Chinese PLA Central Military Commission from Apr.2016 to Oct.2018.All patients were divided,according to results of coronary angiography reexamination after PCI for 12 months,into non-ISR(n=91,ISR<50%with vessel diameter within 5 mm)and ISR group(n=73,ISR≥50%with vessel diameter within 5 mm).ISR group was divided further into 1-vessel ISR group(n=28),2-vessel ISR group(n=32)and 3-vessel ISR group(n=13).The level of serum LncRNA GAS5 was detected by using RT-PCR in all groups.Results The level of serum LncRNA GAS5 was lower in ISR group than that in non-ISR group(P<0.05),and decreased in turn in 1-vessel ISR group,2-vessel ISR group and 3-vessel ISR group(P<0.05).The results of Spearman correlation analysis showed that LncRNA GAS5 level was negatively correlated to ISR vessel numbers(P<0.05).The results of ROC analysis showed that the area under ROC curve was 0.866,cutoff value was 1.099,sensitivity was 86.30%and specificity was 73.60%of LncRNA GAS5 level in ISR diagnosis in elderly patients with CHD after PCI.Conclusion The level of serum LncRNA GAS5 is lower in elderly CHD patients with ISR after PCI,and it is negatively correlated to ISR vessel numbers.The level of serum LncRNA GAS5 has a certain diagnostic value to ISR,and may provide a new idea for therapeutic target in elderly CHD patients with ISR after PCI.
作者
尹鲁平
张洪明
谈红
李宁
王涛
任昌振
刘科卫
Yin Luping;Zhang Hongming;Tan Hong;Li Ning;Wang Tao;Ren Changzhen;Liu Kewei(Cadre Ward,960th Hospital,Chinese PLA Central Military Commission,Jinan,250000,China;不详)
出处
《中国循证心血管医学杂志》
2020年第10期1176-1178,1184,共4页
Chinese Journal of Evidence-Based Cardiovascular Medicine
基金
军委后勤保障局卫生局面上项目(16BJZ51)。
