摘要
目的:分析川芎传统道地产区及沿经度向南连续扩展区域的土壤元素分异特征,从土壤无机元素层面寻找道地川芎产区的自然环境边界。方法:采用X射线荧光光谱法(XRF)及ICP-MS等技术分别测定土壤及川芎植株中24种元素的含量,利用spearman相关检验分析根际土壤与川芎各部位间无机元素的相关性,筛选"特征元素";基于全元素及"特征元素"的Bray-Curtis相似性指数对土壤样本分别进行层次聚类分析,确定与川芎传统道地产区土壤元素特征相似的样地范围。结果:测定元素中,K,Mg,Mn,Rb元素在根际土壤与川芎植物中明显相关。全元素及4种特征元素的地理分异特征均显示川芎传统道地产区与其临近扩展区域S1~S6(除S4)样地土壤元素分布呈现高度相似性;S14~S16样地与传统道地产区的相似度次之;扩展区域中段S7~S13样地与传统道地产区呈现较大差异。结论:研究区域内土壤元素特征不完全受地理距离控制,沿经度呈现"断层式"分布格局。此研究为川芎的栽培适宜区的确立提供了一定的理论依据,其道地产区的自然边界或可由传统的都江堰产区向南延伸60公里至邛崃境内。此外,K,Mg,Mn,Rb特征元素或可作为评估川芎适宜土壤环境的潜在标记因子。
Objective:To find the natural environmental boundary of the origin of the authentic Ligusticum chuanxiong by analyzing the distribution characteristics of soil elements in the traditional geoauthentic area and the southern continuous expanding areas along the same longitude.Method:The contents of24 elements both in soil and plant L.chuanxiong samples were determined by using X-ray fluorescence spectrometry(XRF)and ICP-MS,etc.Spearman correlation test was used to evaluate the correlations of rhizosphere soil and each portion of L.chuanxiong,to screen out"characteristic elements".Bray-Curtis similarity indexes based on all elements and"characteristic elements"were used for hierarchical cluster analysis of soil samples,to identify the sample range with similar soil element charateristics to the traditional geo-authentic area of L.chuanxiong.Result:K,Mg,Mn and Rb elements were significantly correlated in rhizosphere soil and plant L.chuanxiong.Geographical differentiation of all elements and four characteristic elements showed that there was a highest similarity between geo-authentic area and its neighbor plots S1-S6(except plot S4)in soil elements distribution,followed by plots S14-S16.The middle section plots S7-S13 in the expansion areas were quite different from the traditional geo-authentic production areas.Conclusion:The soil element characteristics in the study areas were not completely controlled by geographical distance,but demonstrated the"fault"variation in the areas along longitude.This study provides a theoretical basis for the detrrmination of suitable cultivation area for Ligusticum Chuanxiong,and the natural boundary of its geo-authentic area may be extended an additional 60 km southward along longitude beyond the traditional origin.Additionally,K,Mg,Mn and Rb characteristic elements may be the potential markers to evaluate the suitable soil environment for cultivating L.chuanxiong.
作者
方千
严铸云
吕宏阳
贾红梅
张秫华
王海
何冬梅
FANG Qian;YAN Zhu-yun;LYU Hong-yang;JIA Hong-mei;ZHANG Shu-hua;WANG Hai;HE Dong-mei(Chengdu University of Traditional Chinese Medicine(TCM),Key Laboratory of Standardization of Traditional Chinese Medicinal Herbs Co-sponsored by Province and Ministry of Education,State Key Laboratory and Breeding Base of Systematic Research,Development and Utilization of TCM Resource Co-sponsored by Province and Ministry of Education,Chengdu 611137,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第18期145-153,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81673553)。
关键词
川芎
土壤
无机元素
道地产区
自然边界
Ligusticum chuanxiong
cultivated soil
inorganic elements
geo-authentic area
natural boundary
