摘要
以马铃薯新品种“泉云4号”为试验材料,以未添加激素的MS基础培养基为对照,研究在马铃薯MS基础培养基中添加不同外源激素6-BA和NAA,不同有机添加物番茄、马铃薯、香蕉对马铃薯组培苗生长和结薯的影响,探求组培苗增殖、结薯的最适培养基配方,为规模化生产组培苗和试管薯提供理论依据。结果表明,6-BA在低浓度时对组培苗的长高、结薯有促进作用,高浓度下有抑制作用,但有利于增加叶片数;NAA在低浓度下有利于组培苗的长高、叶片数的增加,在高浓度下有利于结薯,且2种激素之间存在一定的交互作用。最适于组培苗增殖的激素配比为2.00 mg/L 6-BA+0.01 mg/L NAA,最适于结薯的激素配比为2.00 mg/L 6-BA+1.00 mg/L NAA。最适宜组培苗长高和结薯的添加物均为100 g/L马铃薯;最适宜叶片生长的添加物为200 g/L番茄。对于马铃薯组培苗结薯和叶片数的增加,激素和添加物均可以有效促进,但对于长高,添加物的表现更好。
The new potato variety“Quanyun No.4”was used as experimental material,and the MS basic medium without hormone was used as control,the effects of different exogenous hormones 6-BA and NAA,different organic additives tomato,potato,banana on the growth and tuber-setting of potato plantlets in vitro were studied in order to provide basis for large-scale production of tissue culture plantlets and tubers.The results showed that 6-BA promoted the growth of plantlets and the number of leaves at low concentration,and NAA decreased the growth of plantlets and the number of leaves at high concentration,but increased the number of leaves at low concentration,at high concentrations,it was good for tubers,and there was a certain interaction between 6-BA and NAA.The optimum hormone ratio for plantlet multiplication was 2.00 mg/L 6-BA+0.01-0.10 mg/L NAA.The optimum hormone ratio for tuber formation was 2.00 mg/L 6-BA+1.00 mg/L NAA.The most suitable additives for the growth of plantlets and tubers were 100 g/L potatoes and 200 g/L tomatoes.Hormones and additives could effectively promote the growth of potato plantlets and increase the number of leaves,but the performance of additives was better for the growth.
作者
李扬
凌永胜
林金秀
LI Yang;LING Yong-sheng;LIN Jin-xiu(Quanzhou Agricultural Science Research Institute,Quanzhou,Fujian 362212)
出处
《安徽农业科学》
CAS
2020年第21期39-42,46,共5页
Journal of Anhui Agricultural Sciences
基金
福建省重大基金项目(2017NZ0002)
福建省科技保障性项目(2018N0201)。
