摘要
新疆一枝蒿是维吾尔族、哈萨克族的传统常用药,具有活血解毒、去积滞、清热消食等功效。目前,新疆一枝蒿的化学成分尚未被深入阐明,很大程度上制约了该药体内药效物质的系统阐明和质量标准的制定。该文利用HPLC-IT-TOF-MS对新疆一枝蒿中的化学成分进行定性分析,从新疆一枝蒿75%乙醇提取物中共检测到124个化合物,通过与对照品比对,准确鉴定了7个绿原酸类、4个黄酮类和1个倍半萜类化合物;通过匹配多级质谱信息与文献数据以及质谱裂解规律的推导,初步鉴定了20个倍半萜类、65个黄酮类和23个绿原酸类化合物。该文对新疆一枝蒿的化学成分进行全面解析,为新疆一枝蒿的体内药效物质及质量标准的建立提供了有效信息。
Artemisia rupestris is widely used as a folk medicine of Uygur and Kazak with blood and detoxification,deaccumulation stagnation,clearing heat digestion and other effects.Currently,the chemical profile of A.rupestris has not been disclosed,resulting in a great obstacle for the systematic clarification of the efficacy materials and the quality evaluations.In this paper,HPLC-IT-TOF-MS was deployed to characterize the chemical constituents in A.rupestris.As a result,a total of 124 compounds were detected in 75%ethanol extract of A.rupestris.By comparing with the reference compounds,seven chlorogenic acids,and four flavonoids as well as one sesquiterpenoid were definitely identified.Moreover,twenty sesquiterpenes,sixty-five flavonoids and twenty-three chlorogenic acids were preliminarily identified by matching MS/MS spectral information with literature data and applying those empirical mass spectrometric cracking rules.In current study,the chemical composition of A.rupestris was profiled in depth,and the findings are envisioned to provide a theoretical basis for the further studies of this well-known herbal medicine,such as efficacy material characterization and quality assessment.
作者
张佩杰
曹妍
张珂
宋月林
李军
唐丽
ZHANG Pei-jie;CAO Yan;ZHANG Ke;SONG Yue-lin;LI Jun;TANG Li(Key Laboratory of Ethnomedicine(Mitizu University of China),Ministory of Education,School of Pharmacy,Minzu University of China,Beijing 100081,China;Modem Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第19期4658-4666,共9页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81873397)
民族医药教育部重点实验室课题(KLEM-ZZ201803)。
