摘要
为控制生鲜乳质量,加强入厂防控,对生牛乳β-内酰胺酶杯碟法关键步骤开展研究,提高检测准确性。采用微生物杯碟法测定生鲜乳中β-内酰胺酶,使用对青霉素类药物敏感的藤黄微球菌,利用舒巴坦特异性抑制β-内酰胺酶的活性,同时加入青霉素作对照,比对加入舒巴坦与未加入舒巴坦的样品产生的抑菌圈的大小来间接测定样品中是否含有β-内酰胺酶类药物。结果表明:不同来源藤黄微球菌活化最优时间不同;不同品牌β-内酰胺酶酶活力不同对实验结果有直接影响;青霉素标准溶液低温贮存时间最优为0~2 h;供试检测平板的最优倾注量为10~15 mL;供试检测平板冷藏贮存的最优时间为0~1 d。
In order to control the quality of raw milk,and strengthen the prevention and control of entering the factory,the key steps of β-lactamase cylinder plate method were studied to improve the detection accuracy.In the experiment,β-lactamases in raw milk were determined by microbiological cylinder plate method,Micrococcus luteus,which was susceptible to penicillin,was used.Since the vitality of β-lactamases can be inhibited by sulbactam,the β-lactamases-type drugs were indirectly determined by comparing the size of the inhibition zone between samples adding β-lactamases inhibitor and samples without the inhibitor with penicillin as an indicator.The results showed that the optimal activation time of Micrococcus luteus from different sources was different;the different brand β-lactamase activity had a direct impact on the experimental results;the optimal low-temperature storage time of penicillin standard solution was 0~2 h;the optimal pouring amount of test plate was 10~15 mL;the optimal cold storage time of test plate was 0~1 d.
作者
屈雅莉
郭星
张影
肖淑珍
刘丽
刘文雅
张耀广
柴艳兵
李飞
李兴佳
刘志楠
QU Yali;GUO Xing;ZHANG Ying;XIAO Shuzhen;LIU Li;LIU Wenya;ZHANG Yaoguang;CHAI Yanbing;LI Fei;LI Xingjia;LIU Zhinan(Shijiazhuang Junlebao Dairy Co.Ltd.,Shijiazhuang 050021,China)
出处
《中国乳品工业》
CAS
北大核心
2020年第10期52-56,共5页
China Dairy Industry
基金
国家重点研发计划项目(2018YFC1604302)。
关键词
生鲜乳
Β-内酰胺酶
杯碟法
Raw milk
β-lactamases
Cylinder plate method
