摘要
目的研究三乙酰白藜芦醇(triacetyl resveratrol,TRES)诱导白血病细胞HL-60凋亡的作用。方法采用Alamar blue法检测不同浓度(5、12.5、25、50、75μmol/L)TRES在不同时间点(12、24、48 h)对HL-60细胞增殖抑制率,Annexin V-FITC/PI双染色流式法测定不同浓度(5、25、50μmol/L)TRES作用于细胞12 h和24 h时凋亡的影响。Western blotting法检测5、25、50μmol/L TRES作用于细胞24 h时凋亡相关蛋白(STAT3、pSTAT3、Bcl-2、Bax、Cleaved caspase-3)。结果 Alamar blue实验结果表明TRES可以抑制HL-60细胞的增殖,Annexin V-FITC/PI双染色流式法结果显示TRES有诱导HL-60细胞凋亡的作用。Western blotting法检测凋亡相关蛋白的表达,随着TRES浓度升高,STAT3和Bax蛋白表达量无明显变化,pSTAT3表达量显著下调,Bcl-2表达显著下调,Cleaved caspase-3表达显著上调,Bcl-2/BAX比例下调,最终导致细胞凋亡。结论 TRES在体外抑制HL-60细胞增殖,并通过STAT3-Bcl-2/Bax-caspase-3通路诱导HL-60凋亡。
Objective To explore the effect of triacetyl resveratrol(TRES) on apoptosis of human promyelocytic leukemia HL-60 cells. Methods After treatment with TRES in different concentrations(5, 12.5, 25, 50, 75 μmol/L) for indicated times(12, 24, 48 h), the proliferation of HL-60 cells were analyzed by Alamar blue assay. Annexin V-FITC/PI staining were conducted to detect apoptosis induced by TRES(5, 25, 50 μmol/L) for different times(12, 24 h). The expression of STAT3, pSTAT3, Bcl-2, Bax and Cleaved caspase-3 in HL-60 treated by TRES(5, 25, 50 μmol/L) for 24 h were detected by Western blotting. Results The results of Alamar blue showed that TRES had inhibitory effect on HL-60. The Annexin V-FITC/PI double staining showed that TRES induced apoptosis of HL-60 cells. With the increase of TRES concentration, the expression of STAT3 and Bax did not change significantly, the expression of pSTAT3 was significantly down regulated, the expression of Bcl-2 and Cleaved caspase-3 was significantly up-regulated, and the ratio of Bcl-2/Bax was down regulated, which eventually led to apoptosis. Conclusion TRES inhibits HL-60 cell proliferation, and induces apoptosis of HL-60 cells through STAT3-Bcl-2/Bax-caspase-3 pathway.
作者
郑南
徐修才
ZHENG Nan;XU Xiu-cai(Department of Clinical Ijaboratory,the First Affiliated Hospital of University of Science and Technology of China,Hefei,Anhui 230001,China)
出处
《中华全科医学》
2020年第11期1823-1826,共4页
Chinese Journal of General Practice
基金
安徽省自然科学基金(1708085MH223)。
