摘要
目的:探究二陈汤加味对慢性阻塞性肺病(COPD)大鼠血浆、支气管肺泡灌洗液(BALF)中白细胞介素-12(IL-12),γ-干扰素(IFN-γ),IL-9,IL-4和IL-13水平以及对细支气管组织中IL-4受体1(IL-4R1),IL-13受体A1(IL-13RA1)定位表达的影响。方法:将大鼠随机分为正常组,模型组,二陈汤加味低、中、高剂量组,共5组,每组10只。采用香烟烟雾联合脂多糖(LPS)方法制备COPD大鼠模型,二陈汤加味低、中、高剂量组分别以5,10,20 g·kg^-1灌胃(ig),正常组与模型组ig等量生理盐水,连续干预14 d。采用酶联免疫吸附测定(ELISA)检测大鼠血浆,BALF中IL-12,IFN-γ,IL-9,IL-4和IL-13水平,免疫组化(IHC)检测大鼠细支气管组织中IL-4R1,IL-13RA1的定位表达。结果:与正常组比较,模型组血浆BALF中IL-12,IFN-γ水平均显著降低(P<0.01),而IL-9,IL-4和IL-13的水平均显著升高(P<0.01),IL-4R1,IL-13RA1在模型组细支气管组织中的的表达均显著增强(P<0.01);与模型组比较,二陈汤加味中、高剂量血浆和BALF中的IL-12,IFN-γ水平均显著升高(P<0.01),而IL-9,IL-4和IL-13的水平均显著降低(P<0.01),IL-4R1,IL-13RA1在细支气管组织中的的表达均显著减弱(P<0.01)。结论:二陈汤加味可能通过增加IL-12,IFN-γ的释放,减少IL-9,IL-4和IL-13分泌,并抑制IL-4R1和IL-13RA1的表达,起到抗炎和保护细支气管结构的作用。
Objective:To study the effect of modified Erchentang on levels of interleukin-12(IL-12),interferon-γ(IFN-γ),interleukin-9(IL-9),interleukin-4(IL-4)and interleukin-13(IL-13)in plasma and bronchoalveolar lavage fluid(BALF)of all rats, as well as expressions of interleukin-4(IL-4) receptor(IL-4 R1)and interleukin-13(IL-13)receptor(IL-13 RA1)in bronchioles tissue of rats with chronic obstructive pulmonary disease(COPD). Method: Fifty SD rats were randomly divided into 5 groups,namely normal group,model group,and low,middle and high-dose modified Erchentang groups(5,10,20 g·kg^-1),with 10 rats in each group. COPD in rat was prepared by using cigarette smoke combined with dripping lipopolysaccharide(LPS)in trachea. After the modeling,normal and model groups were given normal saline solution through intragastric(ig)administration,while other groups were given corresponding herbal drugs(5,10,20 g·kg^-1)intragastrically(ig)for 14 days. The levels of IL-12,IFN-γ,IL-9,IL-4 and IL-13 in plasma and BALF were detected by Enzyme-linked immunosorbent assay(ELISA)method,and immunohistochemistry(IHC)method was used to detect the expressions of IL-4 R1 and IL-13 RA1 in bronchioles tissue of all of the groups. Result: Compared with the normal group,the levels of IL-12 and IFN-γ were decreased significantly(P<0.01),but the levels of IL-9,IL-4 and IL-13 in plasma and BALF were significantly increased(P<0.01),and the expressions of IL-4 R1 and IL-13 RA1 in bronchioles tissue were increased significantly(P<0.01)in model group. Compared with the model group,the levels of IL-12 and IFN-γ were increased significantly,while the levels of IL-9,IL-4 and IL-13 in plasma and BALF were decreased significantly(P<0.01),and the expressions of IL-4 R1 and IL-13 RA1 in bronchioles tissue were decreased significantly(P<0.01)in modified Erchentang groups(10,20 g·kg^-1). Conclusion:Modified Erchentang has effects in resisting inflammatory and protecting tissue structure of bronchioles. Its mechanism may be correlated with increasing the levels of IL-12,IFN-γ and reducing the levels of IL-9,IL-4 and IL-13 in plasma and BALF,and inhibiting the expressions of IL-4 R1 and IL-13 RA1 in bronchioles tissue.
作者
毛梦迪
尚立芝
徐莉莉
季书
石龙涛
李耀洋
张光远
王国强
谢文英
MAO Meng-di;SHANG Li-zhi;XU Li-li;JI Shu;SHI Long-tao;LI Yao-yang;ZHANG Guang-yuan;WANG Guo-qiang;XIE Wen-ying(Henan University of Chinese Medicine,Zhengzhou 450046,China;The First Clinical School of Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第20期16-24,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金面上项目(81573881)
河南省教育科学“十三五”规划一般课题([2019]-JKGHYB-0101,[2019]-JKGHYB-0114)
国家级大学生创新创业训练计划项目(S201810471013,S201910471010)。
